Anti-Stella抗体
产品名称: Anti-Stella抗体
英文名称: Stella
产品编号: YB--12280R
产品价格: null
产品产地: 中国/美国
品牌商标: Ybscience
更新时间: 2023-08-17T10:29:50
使用范围: 科研使用
上海钰博生物科技有限公司
- 联系人 : 陈环环
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- 邮编 : 200612
- 所在区域 : 上海
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Anti-Stella抗体
产品编号 | YB-12280R |
英文名称 | Stella |
中文名称 | 发育多能性相关蛋白3抗体 |
别 名 | DPPA3; Compaction associated protein 1; Developmental pluripotency associated 3; Developmental pluripotency associated protein 3; Developmental pluripotency-associated protein 3; Dppa 3; Dppa3; DPPA3_HUMAN; PGC7; Stella related protein; Stella-related protein; Stellar. |
规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
说 明 书 | 100ul 200ul |
研究领域 | 肿瘤 发育生物学 干细胞 转录调节因子 表观遗传学 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human, Mouse, |
产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 18kDa |
细胞定位 | 细胞核 细胞浆 |
性 状 | Lyophilized or Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human Stella/DPPA3:101-159/159 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
PubMed | PubMed |
产品介绍 | background: This gene encodes a protein that in mice may function as a maternal factor during the preimplantation stage of development. In mice, this gene may play a role in transcriptional repression, cell division, and maintenance of cell pluripotentiality. In humans, related intronless loci are located on chromosomes 14 and X. [provided by RefSeq, Jul 2008] Function: May play a role in maintaining cell pluripotentiality. Subcellular Location: Nucleus. Cytoplasm. Tissue Specificity: Low expression in testis, ovary and thymus. Expressed in embryonic stem and carcinoma cells. Highly expressed in testicular germ cell tumors. DISEASE: Expressed in fetal ovary. SWISS: Q6W0C5 Gene ID: 359787 Database links: Entrez Gene: 359787 Human Entrez Gene: 73708 Mouse Entrez Gene: 297576 Rat Omim: 608408 Human SwissProt: Q6W0C5 Human SwissProt: Q8QZY3 Mouse SwissProt: Q6IMK0 Rat Unigene: 131358 Human Unigene: 528118 Human Unigene: 27982 Mouse Unigene: 79056 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
产品图片 |
Sample:
Testis (Mouse) Lysate at 40 ug Primary: Anti- Stella (bs-12280R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18 kD Observed band size: 18 kD Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Stella/DPPA3 Polyclonal Antibody, Unconjugated(bs-12280R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |