分子量:39.2 kDa (CD3E), 37.5 kDa (CD3D) 

纯度:>95% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Biotinylated Human CD3E & CD3D by the LAL method.

Buffer:PBS, pH7.4

生物活性:Measured by its binding ability in a functional ELISA.
Immobilized OKT3 mAb at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD3E & CD3D (Cat# CDD-H82W0 ) with a  linear range of 0.02-0.2 μg/mL.

产品特性:Biotinylated Human CD3 epsilon & CD3 delta Protein  has a calculated MW of 39.2 kDa (CD3E), 37.5 kDa (CD3D) . The predicted N-terminus is Asp 23 (CD3E) & Phe 22 (CD3D). As a result of glycosylation, the reducing (R) protein migrates as 40-55 kDa in SDS-PAGE.

产品背景:T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.