内毒素:

Less than 1.0 EU per μg by the LAL method.



纯度:

>95% as determined by SDS-PAGE.



活性:

Measured by its binding ability in a functional ELISA. Immobilized Mouse GITR, His Tag (Catalog # GIR-M5220) at 10μg/mL (100 μL/well) can bind Mouse GITR Ligand, Fc Tag (Catalog # GIL-M526x) with a linear range of 0.007-0.256 μg/mL.



产品背景:

Tumor necrosis factor ligand superfamily member 18 (TNFSF18) is also known as Glucocorticoid - induced TNF-related ligand (GITRL or GITR Ligand), Activation - inducible TNF - related ligand (AITRL), which belongs to the tumor necrosis factor family. TNFSF18 is expressed at high levels in the small intestine, ovary, testis, kidney and endothelial cells. TNFSF18  / GITRL is up-regulated after stimulation by bacterial lipopolysaccharides (LPS). TNFSF18 Can function as costimulator and lower the threshold for T-cell activation and T-cell proliferation. TNFSF18 / GITR Ligand is important for interactions between activated T-lymphocytes and endothelial cells. TNFSF18 also mediates activation of NF-kappa-B.



产品描述:

This protein carries a human IgG1 Fc tag at the N-terminus. The protein has a calculated MW of 41.4 kDa. As a result of glycosylation, the protein migrates as 45 kDa under reducing (R) condition, and 80-100 kDa under non-reducing (NR) condition (SDS-PAGE).
SDS-PAGE


Mouse GITR Ligand / TNFSF18, Fc Tag on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.