ATG5 抗体
Applications
| In-house applications |
ELISA, WB, IHC, IF Application not tested? Please see our Guarantee. |
| Published applications |
IHC, WB |
Publications |
13 citations –view here |
| Species specificity |
Human,Mouse,Rat; other species not tested. |
| Published Species |
Human, mouse, rat |
|
Images
Prev1Next
 mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10181-2-AP(ATG5 antibody) at dilution of 1:1000 Immunohistochemical of paraffin-embedded human colon cancer using 10181-2-AP(ATG5 antibody) at dilution of 1:50 (under 10x lens) IF result of anti-ATG5 (10181-2-AP) with E15 mouse cortical neurons.
|
| Positive WB detected in |
Mouse kidney tissue, human brain tissue, Raji cells |
| Positive IHC detected in |
Human colon cancer tissue |
| Positive IF detected in |
Mouse cortical neurons |
| Recommended dilution |
WB : 1:200-1:2000
IHC : 1:20-1:200
IF : 1:20-1:200 |
ATG5 抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
| Source |
Rabbit |
Purification method |
Antigen affinity purification |
| Isotype |
IgG |
Storage |
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen |
ATG5 fusion protein ag0214 |
Full name |
ATG5 autophagy related 5 homolog (S. cerevisiae) |
Calculated molecular
weight |
32 kDa |
Observed molecular
weight |
50-55kd |
GenBank accession
number |
BC002699 |
Gene ID (NCBI) |
9474 |
| Gene symbol |
ATG5 |
Synonyms |
APG5, APG5 LIKE, APG5L, Apoptosis specific protein, ASP, ATG5, Autophagy protein 5, hAPG5 |
Background ATG5 抗体
|
ATG5, also named as APG5L and ASP, belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection. This antibody is raised against 28-275 amino acids of human ATG5. It can recognize the ATG5-ATG12 complex (55 kDa) and free ATG5 (32 kDa). ATG5 抗体
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