分子量:31.2 kDa (FCGRT), 13.1 kDa (B2M) 

纯度:>95% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg by the LAL method.

Buffer:PBS, pH7.4

生物活性:(1) Measured by its binding ability in a functional ELISA against immobilized Yervoy Ipilimumab (Human IgG1, 1μg/well). The linear range is 0.02-0.15 μg/mL.
(2) Biotinylated Human FcRn / FCGRT & B2M, Avi Tag (Avitag™) (Cat.# FCM-H82W4) coupled to SA coated sensor chip can bind trastuzumab with an affinity constant of 2.2-2.6 μM as determined in a SPR assay (Biacore 2000). The data is generally provided by Biaffin GmbH &Co KG, Germany.

产品特性:The subunit FcRN carries a polyhistidine tag at the C-terminus.
The subunit B2M carries a Strep II tag at the C-terminus. The protein has a calculated MW of 31.2 kDa (FCGRT), 13.1 kDa (B2M) . The protein migrates as 33 kDa (FCGRT), 13 kDa (B2M) respectively on a SDS-PAGE gel under reducing (R) condition due to glycosylation.

产品背景:FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.