分子量:47.4 kDa

纯度:>95% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human 4-1BB Ligand, Fc Tag by the LAL method.

Buffer:50 mM tris, 100 mM glycine, pH7.5

生物活性:(1) Measured by its binding ability in a functional ELISA. 
Immobilized Human 4-1BB, His Tag (Cat# 41B-H5227) at 100 ng/mL (100 l/well),can bind Human 4-1BB Ligand, Fc Tag (Cat# 41L-H5257) with a linear of 0.1-2.5 ng/mL.
(2) Measured by its binding ability in a functional ELISA. 
Immobilized Human 4-1BB Ligand, Fc Tag (Cat# 41L-H5257) at 2μg/mL (100 l/well),can bind Biotinylated Human 4-1BB, Fc Tag (Cat# 41B-H8258) with a linear of 0.5-20 ng/mL.

产品特性:Human 4-1BB Ligand, Fc Tag is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 47.4 kDa. The predicted N-terminus is Ala 50. The reducing (R) protein migrates as 52 kDa in SDS-PAGE .

产品背景:Tumor necrosis factor ligand superfamily member 9 (4-1BBL) is also known as 4-1BB ligand, CD137L or TNFSF9, which is a cytokine that binds to TNFRSF9. 4-1BBL is the high affinity ligand of 4-1BB. 4-1BBL induces the proliferation of activated peripheral blood T-cells. Also, 4-1BBL may have a role in activation-induced cell death (AICD). Furthermore, 4-1BBL may play a role in cognate interactions between T-cells and B-cells/macrophages. As for diseases, 4-1BBL is involved in cancers, infectious diseases and autoimmune diseases.