• 靶标种属Mouse
• 特异性By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, horse, human, pig, rabbit and rat IgG was detected. This antibody may cross react with IgG from other species.
• 经测试应用IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISAmore details
• 最小交叉反应
  Chicken, Cow, Horse, Human, Pig, Rabbit, Ratmore details
• 偶联物Alexa Fluor® 488. Ex: 495nm, Em: 519nm

性能

• 形式Liquid
• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
• 存储溶液Preservative: 0.02% Sodium azide
  Constituents: PBS, 30% Glycerol, 1% BSA
• 浓度200 µg 浓度为 2 mg/ml Batch dependent within range:500 µg 浓度为 1.99 - 2 mg/ml 
  

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• 纯度Immunogen affinity purified
• 纯化说明Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
• 克隆多克隆
• 同种型IgG
• 常规说明

  The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to use products containing Alexa Fluor® dyes for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

• 研究领域
  • Immunology
   
  • Immunoglobulins
   
  • Heavy Chain
   
  • IgG

  • Secondary antibodies
   
  • anti-Mouse
   
  • IgG
   
  • Fluorophore
   
  • Alexa Fluor® 488

应用

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed 图像

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117)

  ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab150117 Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117)This image is courtesy of an Abreview submitted by Kirk McManus.

  ab134375 staining CD24 in human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde. Samples were incubated with primary antibody (1/200 in PBS) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated goat anti-mouse IgG H&L (ab150117) (1/200) was used as the secondary antibody.

  See Abreview

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 488), pre-adsorbed (ab150117)This image is courtesy of Dr. Shaohua Li

  Image: Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical School

  Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)

  Preparation:

  Fix in 3%PFA in PBS for 30 min at RTIncubate in 7.5% sucrose-PBS for 3h at RTIncubate in 15% sucrose-PBS at 4 degree Celsius overnightEmbed the EBs in tissue-Tek OCT compoundCut frozen sections to 4-20 µm thickness

  Primary antibody 1: Mouse anti-Ki67 (ab53280), 1:50

  Primary antibody 2: Rabbit anti-laminin, 1:400
  Secondary antibody 1: Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 488) pre-adsorbed (ab150117), 1:200

  Secondary antibody 2: Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) pre-adsorbed (ab150084), 1:300
  Nuclei were counterstained with DAPI

   

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  Alexa Fluor® - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117)