AAK1,AP2关联激酶1抗体

产品编号HZ-1507R
英文名称AAK1
中文名称AP2关联激酶1抗体
别 名AAK 1; Adaptor associated kinase 1; Aak1; AAK1_HUMAN; Adaptor-associated kinase 1; AP2-associated protein kinase 1; DKFZp686F03202; AP2 associated kinase 1;AP2 associated protein kinase 1; DKFZp686F03202; DKFZp686K16132; FLJ23712; FLJ25931; FLJ31060; FLJ42882; FLJ45252; KIAA1048; MGC138170; MGC164568; MGC164570.
规格价格0.1ml 0.2ml
说 明 书0.1ml 0.2ml
研究领域信号转导 激酶和磷酸酶 转运蛋白
抗体来源Rabbit
AAK1,AP2关联激酶1抗体克隆类型Polyclonal
交叉反应Human, Mouse, Rat,
产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量104kDa
细胞定位细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human AAK1
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
AAK1,AP2关联激酶1抗体PubMedPubMed
产品介绍background:
Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

Function:
Phosphorylates the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2). May play a role in regulating aspects of clathrin-mediated endocytosis.

Subunit:
Interacts with alpha-adaptin, AP-2, clathrin, NUMB andEPS15 isoform 2. Interacts with membrane-bound activated NOTCH1 butnot with the inactive full-length form of NOTCH1. Preferentiallyinteracts with monoubiquitinated activated NOTCH1 compared to thenon-ubiquitinated form.

Subcellular Location:
Cell membrane; Peripheral membrane protein(By similarity). Membrane, clathrin-coated pit (By similarity).Note=Active when found in clathrin-coated pits at the plasmamembrane. In neuronal cells, enriched at presynaptic terminals. Innon-neuronal cells, enriched at leading edge of migrating cells (Bysimilarity).

Tissue Specificity:
Detected in brain, heart and liver. Isoform 1is the predominant isoform in brain.

Post-translational modifications:
Autophosphorylated (By similarity).

AAK1,AP2关联激酶1抗体Similarity:
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 protein kinase domain.

Gene ID:
22848

Database links:
Entrez Gene: 22848 Human
Entrez Gene: 269774 Mouse
Entrez Gene: 500244 Rat
SwissProt: Q2M2I8 Human
SwissProt: Q3UHJ0 Mouse
SwissProt: P0C1X8 Rat
Unigene: 468878 Human
Unigene: 221038 Mouse
Unigene: 203307 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.