phospho-eIF4EBP1（Thr70）,磷酸化eIF4E结合蛋白抗体

产品编号HZ-14550R
英文名称phospho-eIF4EBP1（Thr70）
中文名称磷酸化eIF4E结合蛋白抗体
别 名eIF4EBP1 (phospho T70); eIF4EBP1 (phospho Thr70); p-eIF4EBP1 (Thr70); eIF4EBP1; Eukaryotic translation initiation factor 4E binding protein 1; Eukaryotic translation initiation factor 4E-binding protein 1; 4E BP1; 4EBP1; BP 1; BP1; eIF4E binding protein 1; eIF4E-binding protein 1; Eukaryotic translation initiation factor 4E binding protein 1; MGC4316; PHAS I; PHASI; PHAS-I; PHAS; 4E-BP1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; Phosphorylated heat and acid stable protein regulated by insulin 1; 4EBP1_HUMAN.
说 明 书0.1ml
产品类型磷酸化抗体
研究领域细胞生物 转运蛋白 新陈代谢 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Sheep,
phospho-eIF4EBP1（Thr70）,磷酸化eIF4E结合蛋白抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量12kDa
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human EIF4B around the phosphorylation site of Thr70
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
phospho-eIF4EBP1（Thr70）,磷酸化eIF4E结合蛋白抗体PubMedPubMed
产品介绍background:
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008].

Function:
Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.

Subunit:
Hypophosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) or mTORC1 phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Interacts with RPTOR.

Subcellular Location:

Post-translational modifications:
Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR.

DISEASE:

phospho-eIF4EBP1（Thr70）,磷酸化eIF4E结合蛋白抗体Similarity:
Belongs to the eIF4E-binding protein family.

Gene ID:
1978

Database links:
Entrez Gene: 1978 Human
Entrez Gene: 13685 Mouse
Entrez Gene: 116636 Rat
Omim: 602223 Human
SwissProt: Q13541 Human
SwissProt: Q60876 Mouse
SwissProt: Q62622 Rat
Unigene: 411641 Human
Unigene: 6700 Mouse
Unigene: 11161 Rat

phospho-eIF4EBP1（Thr70）,磷酸化eIF4E结合蛋白抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.