活性重组蛋白人源CD130 / IL6ST / CDW130 / GP130 / IL-6RB
产品名称： 活性重组蛋白人源CD130 / IL6ST / CDW130 / GP130 / IL-6RB
英文名称： Recombinant Protein Human CD130 / IL6ST / CDW130 / GP130 / IL-6RB
产品编号： GX0087
产品价格： 0
产品产地： 武汉
品牌商标： 瑾萱生物
更新时间： null
使用范围： null
武汉瑾萱生物科技有限公司
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纯度
> 95 % as determined by SDS-PAGE.
内毒素
< 0.1 EU per μg of the protein as determined by the LAL method
生物活性
Measured by its ability to inhibit IL-6-dependent proliferation of the 7TD1 mouse hybridoma cells in the presence of Recombinant Human IL-6 R alpha . The ED50 for this effect is 0.65-6.5 ng/mL.
蛋白构建
A DNA sequence encoding the extracellular domain (Met 1-Ile 618) of human IL6ST (NP_002175.2) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Accession Number（Uniprot ID）.
表达宿主
HEK293 Cells
种属
Human
预测 N 端
Glu 23
分子量
The recombinant human IL6ST/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 844 amino acids and has a predicted molecular mass of 95 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh IL6ST/Fc monomer is approximately 125-150 kDa due to glycosylation.
缓冲液
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.