分子量:16.7 kDa

纯度:>95% as determined by reduced SDS-PAGE. 

内毒素:Less than 1.0 EU per μg of the Biotinylated Human VEGF121, His Tag by the LAL method.

Buffer:PBS, pH7.4

生物活性:

产品特性:This protein carries a 15 AA fragment at the N-terminus, followed by a polyhistidine tag. There is one single lysine residue within the 15 AA fragment that is enzymatically labeled with biotin in vivo. The protein has a calculated MW of 16.7 kDa. The reducing (R) protein migrates as 18 kDa and 22 kDa and the no-reducing (NR) protein migrates as 35-40 kDa in SDS-PAGE due to glycosylation.

产品背景:Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, and  is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. Alternatively spliced isoforms of 121,145,165,183,189 and 206 amino acids in length are expressed in humans. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189. VEGF121 is the only form that lacks a basic heparinbinding region and is freely diffusible. Mouse embryos expressing only the corresponding isoform (VEGF120) do not survive to term, and show defects in skeletogenesis. Human VEGF121 shares 87% aa sequence identity with corresponding regions of mouse and rat, 93% with feline, equine and bovine, and 91%, 95% and 96% with ovine, canine and porcine VEGF, respectively. VEGF121 induces the proliferation of lymphatic endothelial cells. The lymphangiogenesis may be promoted by upregulation of VEGF121, which may in turn act in part via induction of VEGF-C.