EAAT2,胶质细胞谷氨酸运载蛋白2抗体

产品编号HZ-20325R
英文名称EAAT2
中文名称胶质细胞谷氨酸运载蛋白2抗体
别 名EAAT2; Excitatory amino acid transporter 2; Excitotoxic amino acid transporter 2; Glial high affinity glutamate transporter; GLT 1; GLT1; Glutamate aspartate transporter II; SLC1A2; Sodium dependent glutamate aspartate transporter 2; Solute carrier family 1 glial high affinity glutamate transporter member 2; Solute carrier family 1 member 2; Excitatory amino acid transporter 2; GLT-1; SLC1A2; Sodium-dependent glutamate/aspartate transporter 2; Solute carrier family 1 member 2.
说 明 书0.1ml 0.2ml
研究领域免疫学 神经生物学 通道蛋白
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Sheep,
EAAT2,胶质细胞谷氨酸运载蛋白2抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量62kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human EAAT2
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
EAAT2,胶质细胞谷氨酸运载蛋白2抗体PubMedPubMed
产品介绍background:
This gene encodes a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Mutations in and decreased expression of this protein are associated with amyotrophic lateral sclerosis. Alternatively spliced transcript variants of this gene have been identified. [provided by RefSeq, Sep 2010]

Function:
Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Subunit : Homotrimer. Interacts with AJUBA.

Subcellular Location:
Membrane; Multi-pass membrane protein.

Tissue Specificity:
Brain

Post-translational modifications:
Glycosylated.
Palmitoylation at Cys-38 is not required for correct subcellular localization, but is important for glutamate uptake activity.

EAAT2,胶质细胞谷氨酸运载蛋白2抗体Similarity:
Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A2 subfamily.

Gene ID:
6506

Database links:
Entrez Gene: 6506 Human
Entrez Gene: 20511 Mouse
Entrez Gene: 29482 Rat
Omim: 600300 Human
SwissProt: P43004 Human
SwissProt: P43006 Mouse
SwissProt: P31596 Rat
Unigene: 502338 Human
Unigene: 267547 Mouse
Unigene: 371582 Mouse
Unigene: 10240 Rat

EAAT2,胶质细胞谷氨酸运载蛋白2抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EAAT2) Polyclonal Antibody, Unconjugated (HZ-20325R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.