碳酸酐酶ⅤB(CA5B)重组蛋白

YB072Ra011
Recombinant Carbonic Anhydrase VB, Mitochondrial (CA5B)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
碳酸酐酶ⅤB(CA5B)重组蛋白[ PROPERTIES ]
Residues: Cys34~Pro317 (Accession # Q9Y2D0),
with two N-terminal Tags, His-tag and T7-tag. Host: E. coli
Subcellular Location: Mitochondrion.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 1mM DTT, 5% trehalose, 0.05%
sarcosyl and preservative.
Predicted isoelectric point: 6.6
碳酸酐酶ⅤB(CA5B)重组蛋白Predicted Molecular Mass: 36.4kDa
Accurate Molecular Mass: 33kDa as determined by SDS-PAGE reducing conditions.
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Note: The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to
give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
碳酸酐酶ⅤB(CA5B)重组蛋白 STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its
sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- CSLYTCT YKTRNRALHP
LWESVDLVPG GDRQSPINIR WRDSVYDPGL KPLTISYDPA TCLHVWNNGY SFLVEFEDST
DKSVIKGGPL EHNYRLKQFH FHWGAIDAWG SEHTVDSKCF PAELHLVHWN AVRFENFEDA
ALEENGLAVI GVFLKLGKHH KELQKLVDTL PSIKHKDALV EFGSFDPSCL MPTCPDYWTY
SGSLTTPPLS ESVTWIIKKQ PVEVDHDQLE QFRTLLFTSE GEKEKRMVDN FRPLQPLMNR
TVRSSFRHDY VLNVQAKPKP ATSQATP
[ REFERENCES ]
1. Fujikawa-Adachi K., et al. (1999) J. Biol. Chem. 274:21228-21233. 2. Koehler K., et al. (2007) Angew. Chem. Int. Ed. Engl. 46:7697-7699. 3. Temperini C., et al. (2007) Bioorg. Med. Chem. Lett. 17:2210-2215. 4. Crocetti L., et al. (2009) Bioorg. Med. Chem. Lett. 19:1371-1375.