分子量:52.1 kDa

纯度:>92% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human PH20, His Tag by the LAL method.

Buffer:50 mM Tris, 100 mM NaCl, pH7.0-7.4

生物活性:

产品特性:Human PH20, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 52.1 kDa. The predicted N-terminus is Leu 36. The reducing (R) protein migrates as 64-66 kDa in SDS-PAGE  due to glycosylation.

产品背景:Hyaluronidase PH-20 is also known as Sperm adhesion molecule 1 (SPAM1) and Sperm surface protein PH-20, which belongs to the glycosyl hydrolase 56 family, SPAM1 / PH-20 is expressed in testis. SPAM-1 / PH20 random hydrolysis of (1->4)-linkages between N  acetyl  beta  D  glucosamine and D-glucuronate residues in hyaluronate. SPAM-1 / PH20 involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. SPAM1 aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.