phospho-arfaptin 2,磷酸化ADP核糖基化因子结合蛋白2抗体

产品编号HZ-3006R
英文名称phospho-arfaptin 2 (Ser260)
中文名称磷酸化ADP核糖基化因子结合蛋白2抗体
别 名ADP ribosylation factor interacting protein 2; Arfaptin 2; ARFIP2; Partner of RAC1; POR1; ARFP2_HUMAN.
规格价格0.1ml
说 明 书0.1ml
产品类型磷酸化抗体
研究领域肿瘤 细胞生物 染色质和核信号 神经生物学 转录调节因子
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Dog, Cow, Rabbit, Sheep,
phospho-arfaptin 2,磷酸化ADP核糖基化因子结合蛋白2抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量38kDa
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human arfaptin 2 around the phosphorylation site of Ser260
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
phospho-arfaptin 2,磷酸化ADP核糖基化因子结合蛋白2抗体PubMedPubMed
产品介绍background:
ARFIP2 is a ubiquitously expressed protein implicated in mediating cross talk between RAC and ARF small GTPases. It has been shown that ARFIP2 binds specifically to GTP-bound ARF1 and ARF6, but binds to Rac-GTP and Rac-GDP with similar affinities. The X-ray structure of arfaptin reveals an elongated, crescent-shaped dimer of 3-helix coiled-coils. Structures of arfaptin with Rac bound to either GDP or the slowly hydrolysable analog GMPPNP show that the switch regions adopt similar conformations in both complexes.

Function:
Putative target protein of ADP-ribosylation factor. Involved in membrane ruffling.

Subunit:
Interacts with RAC1 by binding directly to it. Specifically binds to GTP-bound ARF1 and ARF6, but binds to RAC1.GTP and RAC1.GDP with similar affinities. Directly interacts with ARL1 GTP-bound form; this interaction leads to a > 4-fold increase in the amount of ARL1-bound GTP at steady state level.

Similarity:
Contains 1 AH domain.

Gene ID:
23647

Database links:
Entrez Gene: 23647 Human
Entrez Gene: 76932 Mouse
Entrez Gene: 293344 Rat
Omim: 601638 Human
SwissProt: P53365 Human
SwissProt: Q8K221 Mouse
SwissProt: Q6AY65 Rat
Unigene: 595146 Human
phospho-arfaptin 2,磷酸化ADP核糖基化因子结合蛋白2抗体Unigene: 75139 Human
Unigene: 41637 Mouse
Unigene: 73878 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.