凸素1(PROM1)重组蛋白

YB516Mu013
Recombinant Prominin 1 (PROM1)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
 凸素1(PROM1)重组蛋白[ PROPERTIES ]
Residues: Ile171~Asp431
Tags: N-terminal His-Tag
Accession: O43490
Host: E. coli
Subcellular Location: Cell projection, cilium, photoreceptor outer segment. Apical cell
membrane; Multi-pass membrane protein. Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,
 凸素1(PROM1)重组蛋白150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 6.8
Predicted Molecular Mass: 30.5kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
IGIFYGFVAN HQVRTRIKRS RKLADSNFKD LRTLLNETPE QIKYILAQYN TTKDKAFTDL
 凸素1(PROM1)重组蛋白NSINSVLGGG ILDRLRPNII PVLDEIKSMA TAIKETKEAL ENMNSTLKSL HQQSTQLSSS
LTSVKTSLRS SLNDPLCLVH PSSETCNSIR LSLSQLNSNP ELRQLPPVDA ELDNVNNVLR
TDLDGLVQQG YQSLNDIPDR VQRQTTTVVA GIKRVLNSIG SDIDNVTQRL PIQDILSAFS
VYVNNTESYI HRNLPTLEEY D
[ REFERENCES ]
1. Jaszai J., et al. (2007) Histochem. Cell Biol. 128:409-419. 2. Fargeas C.A., et al. (2007) Tissue Antigens 69:602-606. 3. Huttner H.B., et al. (2008) Stem Cells 26:698-705. 4. Zhang Q., et al. (2007) Hum. Genet. 122:293-299.