R-脊椎蛋白1(RSPO1)重组蛋白

YB171Ra021
Recombinant R-Spondin 1 (RSPO1)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
 R-脊椎蛋白1(RSPO1)重组蛋白[ PROPERTIES ]
Residues: Ser21~Ala135 (Accession # Q2MKA7),
with two N-terminal Tags, His-tag and T7-tag.
Host: E. coli
Subcellular Location: Secreted.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,
500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
 R-脊椎蛋白1(RSPO1)重组蛋白0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 8.9
Predicted Molecular Mass: 16.5kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its
sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SRGIKGKRQR RISAEGSQAC
 R-脊椎蛋白1(RSPO1)重组蛋白AKGCELCSEV NGCLKCSPKL FILLERNDIR QVGVCLPSCP PGYFDARNPD MNKCIKCKIE
HCEACFSHNF CTKCKEGLYL HKGRCYPACP EGSSA
[ REFERENCES ]
1. Parma P., et al. (2006) Nat. Genet. 38:1304-1309. 2. Hao H.X., et al. (2012) Nature 485:195-200. 3. Glinka A., et al. (2011) EMBO Rep. 12:1055-1061. 4. Ruffner H., et al. (2012) PLoS ONE 7:E40976-E40976.