CBX4,染色盒同源物4抗体

产品编号HZ-17376R
英文名称CBX4
中文名称染色盒同源物4抗体
别 名CBX 4; CBX4; Cbx4 chromobox homolog 4 (Drosophila Pc class); CBX4_HUMAN; Chromobox homolog 4 (Pc class homolog, Drosophila); Chromobox homolog 4; Chromobox protein homolog 4; E3 SUMO protein ligase CBX 4; E3 SUMO protein ligase CBX4; E3 SUMO-protein ligase CBX4; hPc 2; hPc2; NBP 16; NBP16; NS5ATP1 binding protein 16; Pc 2; Pc class 2 homolog; Pc class homolog; Pc class homolog Drosophila; PC2; Polycomb 2 homolog.
说 明 书0.1ml 0.2ml
研究领域细胞生物 结合蛋白 表观遗传学 泛素
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Dog, Pig, Horse, Sheep,
CBX4,染色盒同源物4抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量61kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human CBX4
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
CBX4,染色盒同源物4抗体PubMedPubMed
产品介绍background:
Protamines are small, arginine-rich (basic), nuclear proteins that mediate normal sperm head condensation and DNA stabilization (1–3). Mice, humans, and certain fish have 2 or more different protamines, whereas the sperm of bull, boar, rat, rabbit, guinea pig, and ram have one form of protamine (3,4). The majority of DNA in human sperm is bound to protamines with only a small proportion of DNA bound to histones in a way similar to active chromatin (1,2). The retention of histone association with sperm DNA with respect to protamine association to sperm DNA can change within as little as 400 bp of DNA, suggesting that there is fine control over haploid DNA organization (1). Protamines eventually replace histones late in the haploid phase of spermatogenesis (1,2). The human protamine 1 gene maps to chromosome 16p13.13 and encodes a 51 amino acid protein (5). The human protamine 2 gene maps to chromosome 16p13.13 and encodes a 102 amino acid protein (6).

Function:
E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.

Subunit:
Interacts with histone H3-K9Me3. Interacts with CHTOP (By similarity). Component of a PRC1-like complex. Self-associates. Interacts with SUV39H1 and HIPK2. Interacts with CSNK2B.

Subcellular Location:
Nucleus.

Tissue Specificity:
Ubiquitous.

Post-translational modifications:
Phosphorylated on Thr-497 by HIPK2 upon DNA damage; which enhances E3 SUMO-protein ligase activity and promotes sumoylation on Lys-494.

Similarity:
Contains 1 chromo domain.

Gene ID:
8535

Database links:
Entrez Gene: 8535 Human
Entrez Gene: 12418 Mouse
Entrez Gene: 501403 Rat
Omim: 603079 Human
SwissProt: O00257 Human
SwissProt: O55187 Mouse
Unigene: 268070 Mouse
Unigene: 471550 Mouse

CBX4,染色盒同源物4抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.