SPO11,减数分裂重组蛋白SPO11抗体

产品编号HZ-9302R
英文名称SPO11
中文名称减数分裂重组蛋白SPO11抗体
别 名Meiotic recombination protein SPO 11; Meiotic recombination protein SPO11; MGC39953; SPO 11; SPO11 meiotic protein covalently bound to DSB homolog; SPO11 meiotic protein covalently bound to DSB like; SPO11_HUMAN.
说 明 书0.1ml 0.2ml
研究领域肿瘤 细胞生物 细胞周期蛋白 细胞分化 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Horse,
SPO11,减数分裂重组蛋白SPO11抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量45kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human SPO11
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
SPO11,减数分裂重组蛋白SPO11抗体PubMedPubMed
产品介绍background:
Spo11 is a type II topoisomerase that is thought to generate the chromosome breaks that initiate meiotic recombination. The Spo11 protein initiates meiotic recombination by generating DNA double-strand breaks (DSBs) and is required for meiotic synapsis in S. cerevisiae. The DSBs are located mostly in promoter regions, where the chromatin is in an open configuration, and cluster in domains along the chromosome. Expression of the Spo11 is detected mainly in the testis, in agreement with its predicted function in the initiation of meiotic recombination. Disruption of Spo11 leads to severe gonadal abnormalities from defective meiosis and results in infertility.

Function:
Required for meiotic recombination. Mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination (By similarity). Essential for the phosphorylation of SMC3, HORMAD1 and HORMAD2 (By similarity).

Subcellular Location:
Nuclear

SPO11,减数分裂重组蛋白SPO11抗体Tissue Specificity:
Highly expressed in testis.

Similarity:
Belongs to the TOP6A family.

Gene ID:
23626

Database links:
Entrez Gene: 23626 Human
Entrez Gene: 26972 Mouse
Entrez Gene: 100125974 Pig
Entrez Gene: 366261 Rat
Omim: 605114 Human
SwissProt: Q9Y5K1 Human
SwissProt: Q9WTK8 Mouse
Unigene: 159737 Human
Unigene: 23495 Mouse

SPO11,减数分裂重组蛋白SPO11抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.