双丝氨酸/苏氨酸酪氨酸蛋白激酶(DSTYK)重组蛋白

YB527Ra012
Recombinant Dual Serine/Threonine And Tyrosine Protein Kinase (DSTYK)
Organism Species: Rattus norvegicus (Rat)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
 双丝氨酸/苏氨酸酪氨酸蛋白激酶(DSTYK)重组蛋白[ PROPERTIES ]
Residues: Pro650~Leu904
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: Q6XUX2
Host: E. coli
Subcellular Location: Cytoplasm. Cell membrane.
Apical cell membrane. Basolateral cell membrane.
Cell junction.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
 双丝氨酸/苏氨酸酪氨酸蛋白激酶(DSTYK)重组蛋白(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 6.6
Predicted Molecular Mass: 32.6kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
 双丝氨酸/苏氨酸酪氨酸蛋白激酶(DSTYK)重组蛋白Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
P KLGQELGRGQ YGVVYLCDNW GGHFPCALKS VVPPDEKHWN DLALEFHYMR
SLPKHERLVD LHGSVIDYNY GGGSSVAVLL IMERLHRDLY TGLKAGLSLE TRLQIALDVV
EGIRFLHSQG LVHRDIKLKN VLLDKQNRAK ITDLGFCKPEAMMSGSIVGT PIHMAPELFT
GKYDNSVDVY AFGILFWYIC SGSIKLPEAFERCASKDHLW NNVRRGTRPE RLPVFDEECW
QLMEACWDGD PSKRPLLGIV QPIL