CD14,内毒素受体抗体

产品编号HZ-1192R
英文名称CD14
中文名称内毒素受体抗体
别 名CD14 antigen; CD14 molecule; Lipopolysaccharide receptor; LPSR; Monocyte Differentiation Antigen 14; Monocyte differentiation antigen CD14; Myeloid cell specific leucine rich glycoprotein; CD14_MOUSE; Myeloid cell-specific leucine-rich glycoprotein.
说 明 书0.1ml 0.2ml
研究领域转录调节因子 细胞膜受体 糖尿病
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep,
CD14,内毒素受体抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:20-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量40kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from mouse CD14
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
CD14,内毒素受体抗体PubMedPubMed
产品介绍background:
CD14 is a single copy gene encoding 2 protein forms: a 50 to 55 kDa glycosylphosphatidylinositol anchored membrane protein (mCD14) and a monocyte or liver derived soluble serum protein (sCD14) that lacks the anchor. Both molecules are critical for lipopolysaccharide (LPS) dependent signal transduction, and sCD14 confers LPS sensitivity to cells lacking mCD14. Increased sCD14 levels are associated with inflammatory infectious diseases and high mortality in gram negative shock. CD14 also appears to be involved in clearance of gram-negative bacteria via its high affinity binding to LPS-LPB complexes.

Function:
Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.

Subunit:
Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, MD-2 and TLR4.

Subcellular Location:
Cell membrane; Lipid-anchor, GPI-anchor.

Similarity:
Contains 11 LRR (leucine-rich) repeats.

Gene ID:
12475

Database links:
Entrez Gene: 929 Human
Entrez Gene: 12475 Mouse
Omim: 158120 Human
SwissProt: P08571 Human
Unigene: 163867 Human

CD14,内毒素受体抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

CD14又称内毒素受体，属细胞表面糖蛋白家族成员之一。在机体免疫、防御系统引起的一系列反应中介导细胞识别LPS并引起细胞酪氨酸磷酸化、核因子NF-κB转位、触发细胞因子释放和氧自由基产生。CD14在细胞因子产生的调控方面起重要作用,因而对于CD14结构、功能及其临床意义的研究日益受到重视。