CST8,胱抑素8/半胱氨酸蛋白酶抑制剂8抗体

产品编号HZ-9216R
英文名称CST8
中文名称胱抑素8/半胱氨酸蛋白酶抑制剂8抗体
别 名CRES; CST 8; Cst8; CST8_HUMAN; cystatin 8 (cystatin related epididymal specific); Cystatin 8; Cystatin related epididymal specific; Cystatin related epididymal specific protein; Cystatin related epididymal spermatogenic protein; Cystatin related protein epididymis specific; Cystatin-8; Cystatin-related epididymal spermatogenic protein.
说 明 书0.1ml 0.2ml
研究领域细胞生物 免疫学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Dog,
CST8,胱抑素8/半胱氨酸蛋白酶抑制剂8抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量14kDa
细胞定位分泌型蛋白
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human CST8/Cystatin 8
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
CST8,胱抑素8/半胱氨酸蛋白酶抑制剂8抗体PubMedPubMed
产品介绍background:
The cystatin superfamily is a well-established family of cysteine protease inhibitors. All true cystatins inhibit cysteine peptidases of the papain family, such as cathepsins, while some also inhibit legumain family enzymes. The CRES (cystatin-related epididymal spermatogenic) protein defines a new subgroup in the family 2 cystatins of the cystatin superfamily. CRES proteins lack two of the three consensus sites necessary for the cystatin inhibition of C1 cysteine proteases. They are also preferentially expressed in postmeiotic germ cells, the proximal caput epididymidis, and anterior pituitary gonadotrophs. Therefore, CRES proteins may perform unique and tissue-specific functions in the reproductive and neuroendocrine systems. As a member of the CRES subfamily, Cystatin 8 is a 142 amino acid protein that is expressed in the proximal caput region of the epididymis, where it performs a specialized role during sperm development and maturation.

Function:
Performs a specialized role during sperm development and maturation.

Subcellular Location:
Secreted.

Tissue Specificity:
Proximal caput region of the epididymis. Lower expression in the testis. Within the testis it is localized to the elongating spermatids, whereas within the epididymis it is exclusively synthesized by the proximal caput epithelium.

Similarity:
Belongs to the cystatin family.

Gene ID:
10047

CST8,胱抑素8/半胱氨酸蛋白酶抑制剂8抗体Database links:
Entrez Gene: 10047 Human
Entrez Gene: 13012 Mouse
Entrez Gene: 29679 Rat
Omim: 608683 Human
SwissProt: O60676 Human
SwissProt: P32766 Mouse
SwissProt: O88969 Rat
Unigene: 121602 Human
Unigene: 20435 Mouse
Unigene: 18514 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CST8) Polyclonal Antibody, Unconjugated (HZ-9216R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.