Product: Anti-Caspase-1, clone 1H11
Cat. No.: MC-356 (100 μg)
Specificity:
Recognizes an epitope within the p20 fragment
of mouse caspase-1. Detects a band of ~45 kDa
by Western blot. Does not cross-react with
mouse caspase-11 or -12.
Species Reactivity:
Mouse. Other species not tested.
Ig Isotype:
Rat IgG1
Immunogen:
p20 fragment of mouse caspase-1 and synthetic
peptide corresponding to aa 206-220
(TALEMVKEVKEFAAC) of mouse caspase-1.
Format:
100 μg of a 1 mg/mL solution of antibody
purified with protein G sepharose. Antibody in
PBS with 0.02% sodium azide.
Storage:
Store at 4°C for short term and at -20°C long
term. Aliquot to avoid freeze/thaw cycles.
Applications:

Flow cytometry

ELISA

Western Blots
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.
Figure A: Antibody to caspase-1 clone 1H11 (MC-356)
detect overexpressed caspase-1 in 293T cells transiently
transfected with an EE tagged inactive cysteine mutant of
mouse caspase-1 by flow cytometry.
Figure B: Antibody to caspase-1 clone 1H11 (MC-356)
detect overexpressed caspase-1 in 293T cells transiently
transfected with a EE tagged inactive cysteine mutant of
mouse caspase-1 as a band of ~45 kDa.
Figure C: Antibody to caspase-1 clone 1H11 (MC-356)
detect endogenous pro-caspase-1 in spleen from WT mice,
but not from spleen of caspase-1 deficient mice. HSP-70:
loading control.