分子量:17.2 kDa

纯度:>95% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the ActiveMax Mouse VEGF120 by the LAL method.

Buffer:PBS, pH7.4

生物活性:Measured in a cell proliferation assay using human umbilical vein endothelial cells ( HUVEC ). The ED50 for this effect is typically 5 ng/mL.

产品特性:ActiveMax Mouse VEGF120 contains no "tag", and has a calculated MW of 17.2 kDa. The predicted N-terminus is Ala 27. The reducing (R) protein migrates as 18-22 kDa in SDS-PAGE  due to glycosylation.

产品背景:Vascular endothelial growth factor A (VEGFA) is also known as Vascular permeability factor (VPF). VEGFA belongs to the PDGF/VEGF growth factor family. VEGFA is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. VEGFA is produced by a group of three major isoforms as a result of alternative splicing and if any three isoforms are produced (VEGFA120, VEGFA164, and VEGFA188) then this will not result in vessel defects and death of the full VEGFA knockout in mice.