分子量:28.8 kDa

纯度:>95% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human Dkk-1, Strep Tag by the LAL method.

Buffer:PBS, pH7.4

生物活性:

产品特性:Human Dkk-1, Strep Tag is fused with twin strep tag at the C-terminus, and has a calculated MW of 28.8 kDa. The predicted N-terminus is Thr 32 or Ser 35. The reducing (R) protein migrates as 40-50 kDa in SDS-PAGE  due to glycosylation.

产品背景:Members of the dickkopf-related protein family (DKK-1, -2, -3, and -4) are secreted proteins with two cysteine-rich domains separated by a linker region. And DKK1 takes part in embryonic development through its inhibition of the WNT signaling pathway, binds to LRP6 with high affinity and prevents the Frizzled-Wnt-LRP6 complex formation in response to Wnts. DKK1 promotes LRP6 internalization and degradation when it forms a ternary complex with the cell surface receptor Kremen.DKK1 not olny functions as a head inducer during development, but also regulates joint remodeling and bone formation, which suggests roles for DKK1 in the pathogenesis of rheumatoid arthritis and multiple myeloma. More recently research reported, DKK1 impacts eye development from a defined developmental time point on, and is critical for lens separation from the surface ectoderm via β-catenin mediated Pdgfrα and E-cadherin expression.