phospho-ATF2 (Ser490/498),磷酸化活化复制因子2抗体

产品编号HZ-1648R
英文名称phospho-ATF2 (Ser490/498)
中文名称磷酸化活化复制因子2抗体
别 名ATF2 (phospho S490); p-ATF2 (phospho S490); ATF2 (phospho S490 + S498); p-ATF2 (phospho S490 + S498); CREB 2; HB 16;Activating Transcription Factor 2; ATF 2; Atf-2; ATF2 protein; cAMP Response Element Binding Protein 2; cAMP response element binding protein CRE BP1; cAMP-dependent transcription factor ATF-2; cAMP-responsive element-binding protein 2; CRE BP1; CRE-BP; CREB 2; CREB2; CREBP1; Cyclic AMP dependent transcription factor ATF 2; Cyclic AMP-responsive.
规格价格0.1ml
说 明 书0.1ml
产品类型磷酸化抗体
研究领域肿瘤 免疫学 染色质和核信号 信号转导 细胞凋亡 转录调节因子
phospho-ATF2 (Ser490/498),磷酸化活化复制因子2抗体抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Pig, Rabbit, Guinea Pig,
产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量45kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human ATF2 around the phosphorylation site of Ser490/498
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
phospho-ATF2 (Ser490/498),磷酸化活化复制因子2抗体PubMedPubMed
产品介绍background:
ATF2 is a member of the ATF/CREB family of basic region leucine zipper DNA binding proteins that regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes. Many of these genes are important in cell growth and differentiation, and in stress and immune responses. ATF2 is a nuclear protein that binds DNA as a dimer and can form dimers with members of the ATF/CREB and Jun/Fos families. It is a stronger activator as a heterodimer with cJun than as a homodimer. Several isoforms of ATF2 arise by differential splicing. The stable native full length ATF2 is transcriptionally inactive as a result of an inhibitory direct intramolecular interaction of its carboxy terminal DNA binding domain with the amino terminal transactivation domain. Following dimerization ATF2 becomes a short lived protein that undergoes ubiquitination and proteolysis, seemingly in a protein phosphatase-dependent mechanism. Stimulation of the transcriptional activity of ATF2 occurs following cellular stress induced by several genotoxic agents, inflammatory cytokines, and UV irradiation. This activation requires phosphorylation of two threonine residues in ATF2 by both JNK/SAP kinase and p38 MAP kinase. ATF2 is abundantly expressed in brain.

Subunit:
Binds DNA as a dimer and can form a homodimer in the absence of DNA. Can form a heterodimer with JUN. Interacts with SMAD3 and SMAD4. Binds through its N-terminal region to UTF1 which acts as a coactivator of ATF2 transcriptional activity.

Subcellular Location:
Nucleus.

Tissue Specificity:
Abundant expression seen in the brain.

Similarity:
Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Contains 1 C2H2-type zinc finger.

Gene ID:
1386

phospho-ATF2 (Ser490/498),磷酸化活化复制因子2抗体Database links:
Entrez Gene: 1386 Human
Entrez Gene: 100047997 Mouse
Entrez Gene: 11909 Mouse
Entrez Gene: 81647 Rat
Omim: 123811 Human
SwissProt: P15336 Human
SwissProt: P16951 Mouse
SwissProt: Q00969 Rat
Unigene: 592510 Human
Unigene: 209903 Mouse
Unigene: 9825 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.