VANGL2,神经管畸形相关蛋白VANGL2抗体

产品编号HZ-9465R
英文名称VANGL2
中文名称神经管畸形相关蛋白VANGL2抗体
别 名Homolog of Drosophila strabismus; KIAA1215; Loop tail associated protein; loop tail protein 1 homolog; Loop-tail protein 1 homolog; LPP 1; LPP1; LTAP; STB 1; STB1; STBM 1; STBM; STBM1; Strabismus 1; van Gogh like protein 2; Van Gogh-like protein 2; Vang (van gogh, Drosophila) like 2; Vang like 2 (van gogh, Drosophila); vang like 2; vang like protein 2; Vang-like protein 2; VANG2_HUMAN; VANGL 2; Vangl2.
说 明 书0.1ml 0.2ml
研究领域心血管 发育生物学 神经生物学 干细胞
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Cow, Sheep,
VANGL2,神经管畸形相关蛋白VANGL2抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量60kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human VANGL2
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
VANGL2,神经管畸形相关蛋白VANGL2抗体PubMedPubMed
产品介绍background:
The Vang family of proteins are integral membrane proteins that are homologs of the Drosophila tissue polarity gene strabismus. The gene encoding for Van Gogh-like protein 1 (Vangl1), also designated Strabismus 2 (STB2), localizes to chromosome 1p11-p13.1. Van Gogh-like protein 2 (Vangl2), also designated Strabismus1 (STB1), localizes on chromosome 1q22-q23. Vangl1 is expressed in testis and ovary, but also in gastric and pancreatic cancer. Vangl proteins play a key developmental role in establishing planar cell polarity (PCP) and in regulating convergent extension (CE) movements during embryogenesis. Vangl1 and Vangl2 are both down-regulated in several cancer cell lines and primary tumors.
Involved in the control of early morphogenesis and patterning of both axial midline structures and the development of neural plate. Plays a role in the regulation of planar cell polarity, particularly in the orientation of stereociliary bundles in the cochlea. Required for polarization and movement of myocardializing cells in the outflow tract and seems to act via RHOA signaling to regulate this process.

Function:
Involved in the control of early morphogenesis and patterning of both axial midline structures and the development of neural plate. Plays a role in the regulation of planar cell polarity, particularly in the orientation of stereociliary bundles in the cochlea. Required for polarization and movement of myocardializing cells in the outflow tract and seems to act via RHOA signaling to regulate this process (By similarity).

Subunit:
Interacts through its C-terminal region with the N-terminal half of DVL1, DVL2 and DVL3. The PDZ domain of DVL1, DVL2 and DVL3 is required for the interaction. Also interacts with the PDZ domains of MAGI3, SCRIB/SCRB1 and FZD3 (By similarity).

Subcellular Location:
Membrane; Multi-pass membrane protein (Potential).

VANGL2,神经管畸形相关蛋白VANGL2抗体DISEASE:
Defects in VANGL2 are a cause of neural tube defects (NTD) [MIM:182940]. NTD are congenital malformations of the central nervous system and adjacent structures related to defective neural tube closure during the first trimester of pregnancy. Failure of neural tube closure can occur at any level of the embryonic axis. Common NTD forms include anencephaly, myelomeningocele and spina bifida, which result from the failure of fusion in the cranial and spinal region of the neural tube. NTDs have a multifactorial etiology encompassing both genetic and environmental components.

Similarity:
Belongs to the Vang family.

Gene ID:
57216

Database links:
Entrez Gene: 57216 Human
Entrez Gene: 93840 Mouse
Entrez Gene: 289229 Rat
Omim: 600533 Human
SwissProt: Q9ULK5 Human
SwissProt: Q91ZD4 Mouse
SwissProt: P84889 Rat
Unigene: 99477 Human
Unigene: 36148 Mouse
Unigene: 392110 Mouse
Unigene: 198958 Rat

VANGL2,神经管畸形相关蛋白VANGL2抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-VANGL2 Polyclonal Antibody, Unconjugated(HZ-9465R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining