Active牛FGF basic全长蛋白质-蛋白质/抗原/多肽-试剂-生物在线
深圳市宇德立生物科技有限公司
Active牛FGF basic全长蛋白质

Active牛FGF basic全长蛋白质

商家询价

产品名称： Active牛FGF basic全长蛋白质

英文名称： Basic fibroblast growth factor Basic fibroblast growth factor bFGF BFGF FGF 2 FGF B FGF-2 Fgf2 FGF2 basic FGF2_HUMAN FGFB Fibroblast growth

产品编号： ab129471

产品价格： 0

产品产地： 英国

品牌商标： abcam

更新时间： null

使用范围： null

深圳市宇德立生物科技有限公司
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  • 地址 : 深圳市宝安区西乡宝民二路贤基大厦4E
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  • 所在区域 : 广东
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进入展台

 

  • 性质Recombinant
  • 来源E. coli
  • 氨基酸序列
    • AccessionP03969
    • 种属Cow
    • 序列The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Ala-Gly-Ser.
    • 分子量17 kDa
    • 氨基酸1 to 155

技术指标

Our Abpromise guarantee covers the use of ab129471 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性The ED50, measured in a mitogenic assay using quiescent NR6R-3T3 fibroblasts was found to be <0.1 ng/ml, corresponding to a specific activity of 10 MUnits/mg.
  • 应用

    HPLC

    SDS-PAGE

    Functional Studies

  • 纯度>= 97 % by SDS-PAGE.
    ab129471 is purified by proprietary chromatographic techniques. Purity is greater than 97% as determined by HPLC and SDS-PAGE.
  • 形式Lyophilised
  • 补充说明Endotoxin Level: <0.1 ng/µg.
  • 浓度由溶解方法决定 

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at -20ºC.

    Constituent: 1% Human serum albumin

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶Reconstitute in sterile water to a concentration not less than 0.1 mg/ml.

常规信息

  • 别名
    • Basic fibroblast growth factor
    • Basic fibroblast growth factor bFGF
    • BFGF
    see all
  • 功能Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro.
  • 组织特异性Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.
  • 序列相似性Belongs to the heparin-binding growth factors family.
  • 翻译后修饰Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
    Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.
  • 细胞定位Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.