• 经测试应用IHC-P, ICC/IF, WBmore details
• 种属反应性
  与反应: Mouse, Recombinant Fragment
  预测可用于: Human, Chimpanzee, Macaque Monkey, Gorilla, Orangutan
• 免疫原

  Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Mouse beta 2 Microglobulin.

  参阅Abcam的专有抗源政策

   

   

• 阳性对照
  • This antibody gave a positive signal in Beta 2 Microglobulin Recombinant protein as well as the following Mouse tissue lysates: Spleen; Thymus; Bone Marrow; Lung. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: MEF1 IHC-P: FFPE mouse spleen tissue sections

性能

• 形式Liquid
• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
• 存储溶液pH: 7.40
  Preservative: 0.02% Sodium azide
  Constituent: PBS
  Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
• 浓度Batch dependent within range: 100 µg 浓度为 0.3 - 0.4 mg/ml 
  

  查找您批次的浓度
• 纯度Immunogen affinity purified
• 克隆多克隆
• 同种型IgG
• 研究领域
  • Cardiovascular
   
  • Blood
   
  • Serum Proteins

  • Cancer
   
  • Tumor biomarkers
   
  • Globulins

  • Cardiovascular
   
  • Blood
   
  • Blood Cell Antigens
   
  • WBC Antigens
   
  • HLA

应用

靶标

• 功能Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
• 疾病相关Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
  Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis.
• 序列相似性Belongs to the beta-2-microglobulin family.
  Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
• 翻译后修饰Glycation of Ile-21 is observed in long-term hemodialysis patients.
• 细胞定位Secreted. Detected in serum and urine.
• Information by UniProt
• 数据库链接
  • Entrez Gene: 567 Human
  • Entrez Gene: 12010 Mouse
  • Omim: 109700 Human
  • SwissProt: P61769 Human
  • SwissProt: Q6V7J5 Macaque Monkey
  • SwissProt: P01887 Mouse
  • Unigene: 534255 Human
  • Unigene: 702947 Human

  see all
• 别名
  • B2M antibody
  • B2MG_HUMAN antibody
  • Beta 2 microglobin antibody

  see all

Anti-beta 2 Microglobulin antibody 图像

• 

  Western blot - Anti-beta 2 Microglobulin antibody (ab87483)
  All lanes : Anti-beta 2 Microglobulin antibody (ab87483) at 1 µg
  
  Lane 1 : Spleen (Mouse) Tissue Lysate
  Lane 2 : Thymus (Mouse) Tissue Lysate
  Lane 3 : Mouse Bone Marrow Tissue Lysate
  Lane 4 : Lung (Mouse) Whole Cell Lysate - normal tissue (ab29297)
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 13 kDa
  Observed band size : 13 kDa
  Additional bands at : 285 kDa,52 kDa,92 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 16 minutes
• 

  Western blot - Anti-beta 2 Microglobulin antibody (ab87483)
  Anti-beta 2 Microglobulin antibody (ab87483) at 1 µg/ml + Beta 2 Microglobulin Recombinant Protein at 0.1 µg
  
  Secondary
  Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 13 kDa
  Observed band size : 12 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 23 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 1 minute
• 

  Immunocytochemistry/ Immunofluorescence - Anti-beta 2 Microglobulin antibody (ab87483)

  ab87483 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab87483 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• TUNEology 波长可调检测卡盒-->