Lubricin,巨核细胞刺激因子抗体

产品编号HZ-11175R
英文名称Lubricin
中文名称巨核细胞刺激因子/蛋白多糖4/浅表层粘膜蛋白多糖抗体
别 名Superficial zone proteoglycan; articular superficial zone protein; bG174L6.2; CACP; camptodactyly arthropathy coxa vara pericarditis syndrome gene; FLJ32635; HAPO; Jacobs camptodactyly-arthropathy-pericarditis syndrome gene; JCAP; megakaryocyte stimulating factor; MSF; PRG 4; PRG4; proteoglycan 4; Proteoglycan4; Superficial zone proteoglycan; SZP; PRG4_HUMAN.
说 明 书0.1ml 0.2ml
研究领域信号转导 细胞骨架 细胞外基质
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Cow, Rabbit,
Lubricin,巨核细胞刺激因子抗体产品应用ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量152kDa
细胞定位分泌型蛋白
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Lubricin/SZP
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Lubricin,巨核细胞刺激因子抗体PubMedPubMed
产品介绍background:
Lubricin, also designated proteoglycan-4 or megakaryocyte stimulating factor, is important for boundary lubrication within articulating joints. It is a disulfide-linked homodimer (between Cysteine 1146 and Cysteine 1403) that is essential for protein cleavage. Lubricin inhibits synovial cell adhesion to the cartilage surface, but also prevents the deposition of proteins from synovial fluid onto cartilage. Lubricin is highly expressed in cartilage, liver and synovial tissue. Defects in the gene encoding for lubricin can cause Jakobs syndrome, also designated camptodactyly-arthropathy-coxa vara-pericarditis syndrome (CACP). CACP is an autosomal recessive disorder characterized by joint failure associated with noninflammatory synoviocyte hyperplasia and subinitimal fibrosis of the synovial capsule. Lubricin undergoes different levels of glycosylation and may be detected at varying molecular weights.

Function:
Plays a role in boundary lubrication within articulating joints. Prevents protein deposition onto cartilage from synovial fluid by controlling adhesion-dependent synovial growth and inhibiting the adhesion of synovial cells to the cartilage surface.
Isoform F plays a role as a growth factor acting on the primitive cells of both hematopoietic and endothelial cell lineages. soluble molecule that acts as a carrier for insoluble surface-active phospholipid (SAPL). Depletion of lubricin function has been associated with camptodactyly-arthropathy-coxa vara-pericarditis syndrome (CACP), an arthritic-like autosomal recessive disorder.

Subunit:
Homodimer; disulfide-linked.

Subcellular Location:
Secreted.

Tissue Specificity:
Highly expressed in synovial tissue, cartilage and liver and weakly in heart and lung. Isoform B is expressed in kidney, lung, liver, heart and brain. Isoform C and isoform D are widely expressed.

Post-translational modifications:
N-glycosylated.
O-glycosylated; contains glycosaminoglycan chondroitin sulfate and keratan sulfate.
The disulfide bond between Cys-1146 and Cys-1403 is essential for protein cleavage.

Lubricin,巨核细胞刺激因子抗体DISEASE:
Defects in PRG4 are the cause of camptodactyly-arthropathy-coxa vara-pericarditis syndrome (CACP) [MIM:208250]; also known as Jacobs syndrome. CACP is an autosomal recessive disorder. Individuals with CACP have normal appearing joints at birth but with advancing age develop joint failure associated with noninflammatory synoviocyte hyperplasia and subintimal fibrosis of the synovial capsule.

Similarity:
Contains 2 hemopexin-like domains.
Contains 2 SMB (somatomedin-B) domains.

Gene ID:
10216

Database links:
Entrez Gene: 10216 Human
Entrez Gene: 280867 Cow
Entrez Gene: 96875 Mouse
Omim: 604283 Human
SwissProt: Q92954 Human
SwissProt: Q9JM99 Mouse
Unigene: 647723 Human
Unigene: 329131 Mouse

Lubricin,巨核细胞刺激因子抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.