Doppel,朊蛋白DPL抗体

产品编号HZ-11732R
英文名称Doppel
中文名称朊蛋白DPL抗体
别 名DPL; Dublet; MGC41841; Prion gene complex downstream; Prion like protein doppel; Prion protein 2 (dublet); Prion protein 2; Prion-like protein doppel; PRND; PRND_HUMAN; PrPLP.
说 明 书0.1ml 0.2ml
研究领域细胞生物 神经生物学 细菌及病毒
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Pig, Cow,
Doppel,朊蛋白DPL抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量14kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Doppe1 (51-120aa)
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Doppel,朊蛋白DPL抗体PubMedPubMed
产品介绍background:
Prion diseases or transmissible spongiform encephalopathies (TSEs) are manifested as genetic, infectious or sporadic, lethal neurodegenerative disorders involving alterations of the prion protein (PrP). Infectious PrPSc is highly expressed in the brain of animals affected by TSEs, including scrapie in sheep, BSE in cattle, and Cruetzfeldt-Jacob disease in humans. The PRND gene locus, located on human chromosome 20p, encodes for the doppel protein (Dpl), which exhibits approximately 25% sequence homology with PrP. Dpl is characterized by an alpha-helical conformation, intramolecular disulfide bonds, and two N-linked oligosaccharides, and it is presented on the cell surface by a glycosylphosphatidylinositol anchor. Dpl is highly expressed in adult testis and heart and is detectable in the brain of neonatal mice. Dpl does not appear to contribute to prion disease progression, but ectopic expression of Dpl is implicated in neuronal degeneration of ataxic PRP-deficient mice. Dpl is also thought to play a role in angiogenesis, specifically maturation of the blood-brain barrier.

Subcellular Location:
ell membrane; Lipid-anchor, GPI-anchor

Tissue Specificity:
Expressed in testis.

Similarity:
Belongs to the prion family.

Database links:
Entrez Gene: 23627 Human
Entrez Gene: 26434 Mouse
Entrez Gene: 113910 Rat
Omim: 604263 Human
SwissProt: Q9UKY0 Human
SwissProt: Q9QUG3 Mouse
Unigene: 406696 Human
Unigene: 180750 Mouse
Unigene: 94278 Rat

Doppel,朊蛋白DPL抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.