BAX抗体
Applications
| In-house applications |
ELISA, WB, IP, IF, IHC, FC Application not tested? Please see our Guarantee. |
| Published applications |
IF, IHC, WB |
Publications |
94 citations –view here |
| Species specificity |
Human, Mouse, Rat; other species not tested. |
| Published Species |
Guinea pig, human, mouse, rabbit, rat |
|
Images
Prev12Next
 HeLa cells were subjected to SDS PAGE followed by western blot with 50599-2-Ig(BAX antibody) at dilution of 1:2000 IP Result of anti-BAX (IP:50599-2-Ig, 3ug; Detection:50599-2-Ig 1:2000) with Raji cells lysate 3500ug. Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using 50599-2-Ig( BAX Antibody) at dilution of 1:200 (under 10x lens). Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using 50599-2-Ig( BAX Antibody) at dilution of 1:200 (under 40x lens). Immunofluorescent analysis of HEK-293 cells using 50599-2-Ig( BAX Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) 1X10^6 Raji cells were stained with 0.2ug BAX antibody (50599-2-Ig, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
|
| Positive WB detected in |
HeLa cells, COLO 320 cells, HEK-293 cells, mouse kidney tissue, Raji cells, rat kidney tissue, RAW264.7 cells |
| Positive IP detected in |
Raji cells |
| Positive IHC detected in |
Human lymphoma tissue, human gliomas tissue, human testis tissue, mouse brain tissue |
| Positive IF detected in |
HEK-293 cells, HeLa cells |
| Positive FC detected in |
Raji cells, HeLa cells |
| Recommended dilution |
WB : 1:1000-1:10000
IP : 1:1000-1:10000
IHC : 1:20-1:200
IF : 1:10-1:100 |
BAX抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
| Source |
Rabbit |
Purification method |
Protein A purification |
| Isotype |
IgG |
Storage |
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen |
BAX fusion protein ag0576 |
Full name |
BCL2-associated X protein |
Calculated molecular
weight |
21 kDa |
Observed molecular
weight |
21-24 kDa |
GenBank accession
number |
BC014175 |
Gene ID (NCBI) |
581 |
| Gene symbol |
BAX |
Synonyms |
Apoptosis regulator BAX, BAX |
Background BAX抗体
|
BAX, also named as BCL2L4, is a pro-apoptotic member of the Bcl-2 protein family, which plays a pivotal role in controlling cell life and death. Bax largely localizes to the cytoplasm of healthy cells, but accumulates on the outer mitochondrial membrane upon apoptosis induction (PMID: 9108035). BAX can commit a cell to apoptosis by translocation from the cytosol to the mitochondria and permeabilization of the outer mitochondrial membrane, which leads to the release of cytochrome c from mitochondria (PMID: 21763611). The expression of BAX is upregulated by the tumor suppressor protein p53, and BAX has been shown to be involved in p53-mediated apoptosis (PMID: 8183579). BAX抗体
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