卵磷脂胆固醇脂酰转移酶(LCAT)重组蛋白

YB516Ra021
Recombinant Lecithin Cholesterol Acyltransferase (LCAT)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
 卵磷脂胆固醇脂酰转移酶(LCAT)重组蛋白[ PROPERTIES ]
Residues: Ile290~Ser433
Tags: N-terminal His-Tag
Accession: P04180
Host: E. coli
Subcellular Location: Secreted. Purity: >90%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 1mM DTT, 5% trehalose, 0.05%
s 卵磷脂胆固醇脂酰转移酶(LCAT)重组蛋白arcosyl and preservative.

Predicted isoelectric point: 5.8
Predicted Molecular Mass: 17.7kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
 卵磷脂胆固醇脂酰转移酶(LCAT)重组蛋白precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
I STPSFNYTGR DFQRFFADLH FEEGWYMWLQ SRDLLAGLPA PGVEVYCLYG VGLPTPRTYI
YDHGFPYTDP VGVLYEDGDD TVATRSTELC GLWQGRQPQP VHLLPLHGIQ HLNMVFSNLT
LEHINAILLG AYRQGPPASP TAS
[ REFERENCES ]
1. McLean J., et al. (1986) Nucleic Acids Res. 14:9397-9406. 2. McLean J., et al. (1986) Proc. Natl. Acad. Sci. U.S.A. 83:2335-2339. 3. Tata F., et al. (1987) Biochim. Biophys. Acta 910:142-148. 4. Rogne S., et al. (1987) Biochem. Biophys. Res. Commun. 148:161-169.