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pEX-18Tc载体图谱：
pEX-18Tc载体序列
Cloning vector pEX18Tc, complete sequence
 
LOCUS       AF047519                6349 bp    DNA     circular SYN 25-NOV-2003 
DEFINITION  Cloning vector pEX18Tc, complete sequence. 
ACCESSION   AF047519 
VERSION     AF047519.1  GI:2961144 
KEYWORDS    . 
SOURCE      Cloning vector pEX18Tc 
  ORGANISMCloning vector pEX18Tcother sequences; artificial sequences; vectors. 
REFERENCE   1  (bases 1 to 6349) 
  AUTHORS   Hoang,T.T., Karkhoff-Schweizer,R.R., Kutchma,A.J. and 
            Schweizer,H.P. 
  TITLE     A broad-host-range Flp-FRT recombination system for site-specific 
            excision of chromosomally-located DNA sequences: application for 
            isolation of unmarked Pseudomonas aeruginosa mutants 
  JOURNAL   Gene 212 (1), 77-86 (1998) 
   PUBMED9661666REFERENCE   2  (bases 1 to 6349) 
  AUTHORS   Hoang,T.T., Karkhoff-Schweizer,R.R., Kutchma,A.J. and 
            Schweizer,H.P. 
  TITLE     Direct Submission 
  JOURNAL   Submitted (10-FEB-1998) Microbiology, Colorado State University, 
            Fort Collins, CO 80523, USAFEATURES             Location/Qualifierssource          1..6349 
                     /organism="Cloning vector pEX18Tc" 
                     /mol_type="genomic DNA" 
                     /db_xref="taxon:73160"genecomplement(209..1630) 
                     /gene="sacB"CDScomplement(209..1630) 
                     /gene="sacB" 
                     /codon_start=1 
                     /transl_table=11/product="levansucrase precursor" 
                     /protein_id="AAC27026.1" 
                     /db_xref="GI:2961145" 
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGIS 
                     HITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHG 
                     YHIVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQT 
                     QEWSGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIF 
                     DGDGKTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEES 
                     LFNKAYYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIAS 
                     NTVTDEIERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLN 
                     KTGLVLKMDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLN 
                     IKGKKTSVVKDSILEQGQLTVNK"oriT2104..2928terminatorcomplement(2980..3006) 
                     /note="T2"terminatorcomplement(3139..3182) 
                     /note="T1"rRNAcomplement(3186..3305) 
                     /product="Eschericia coli 5S ribosomal RNA"genecomplement(3341..3625) 
                     /gene="lacZalpha"CDScomplement(3341..3625) 
                     /gene="lacZalpha" 
                     /codon_start=1 
                     /transl_table=11/product="lac alpha peptide" 
                     /protein_id="AAC27027.1" 
                     /db_xref="GI:2961146" 
                     /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLN 
                     RLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRAWLFWRMREDFQPDTD"genecomplement(4885..6075) 
                     /gene="tet"CDScomplement(4885..6075) 
                     /gene="tet" 
                     /codon_start=1 
                     /transl_table=11/product="tetracycline efflux protein" 
                     /protein_id="AAC27028.1" 
                     /db_xref="GI:2961147" 
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH 
                     YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG 
                     RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH 
                     APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV 
                     FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE 
                     KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG 
                     QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR 
                     ATST"ORIGIN1tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca61cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg121ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc181accataatcg gcattttctt ttgcgttttt atttgttaac tgttaattgt ccttgttcaa241ggatgctgtc tttgacaaca gatgttttct tgcctttgat gttcagcagg aagctaggcg301caaacgttga ttgtttgtct gcgtagaatc ctctgtttgt catatagctt gtaatcacga361cattgtttcc tttcgcttga ggtacagcga agtgtgagta agtaaaggtt acatcgttag421gatcaagatc catttttaac acaaggccag ttttgttcag cggcttgtat gggccagtta481aagaattaga aacataacca agcatgtaaa tatcgttaga cgtaatgccg tcaatcgtca541tttttgatcc gcgggagtca gtgaacagat accatttgcc gttcatttta aagacgttcg601cgcgttcaat ttcatctgtt actgtgttag atgcaatcag cggtttcatc acttttttca661gtgtgtaatc atcgtttagc tcaatcatac cgagagcgcc gtttgctaac tcagccgtgc721gttttttatc gctttgcaga agtttttgac tttcttgacg gaagaatgat gtgcttttgc781catagtatgc tttgttaaat aaagattctt cgccttggta gccatcttca gttccagtgt841ttgcttcaaa tactaagtat ttgtggcctt tatcttctac gtagtgagga tctctcagcg901tatggttgtc gcctgagctg tagttgcctt catcgatgaa ctgctgtaca ttttgatacg961tttttccgtc accgtcaaag attgatttat aatcctctac accgttgatg ttcaaagagc1021tgtctgatgc tgatacgtta acttgtgcag ttgtcagtgt ttgtttgccg taatgtttac1081cggagaaatc agtgtagaat aaacggattt ttccgtcaga tgtaaatgtg gctgaacctg1141accattcttg tgtttggtct tttaggatag aatcatttgc atcgaatttg tcgctgtctt1201taaagacgcg gccagcgttt ttccagctgt caatagaagt ttcgccgact ttttgataga1261acatgtaaat cgatgtgtca tccgcatttt taggatctcc ggctaatgca aagacgatgt1321ggtagccgtg atagtttgcg acagtgccgt cagcgttttg taatggccag ctgtcccaaa1381cgtccaggcc ttttgcagaa gagatatttt taattgtgga cgaatcgaac tcaggaactt1441gatatttttc atttttttgc tgttcaggga tttgcagcat atcatggcgt gtaatatggg1501aaatgccgta tgtttcctta tatggctttt ggttcgtttc tttcgcaaac gcttgagttg1561cgcctcctgc cagcagtgcg gtagtaaagg ttaatactgt tgcttgtttt gcaaactttt1621tgatgttcat cgttcatgtc tcctttttta tgtactgtgt tagcggtctg cttcttccag1681ccctcctgtt tgaagatggc aagttagtta cgcacaataa aaaaagacct aaaatatgta1741aggggtgacg ccaaagtata cactttgccc tttacacatt ttaggtcttg cctgctttat1801cagtaacaaa cccgcgcgat ttacttttcg acctcattct attagactct cgtttggatt1861gcaactggtc tattttcctc ttttgtttga tagaaaatca taaaaggatt tgcagactac1921gggcctaaag aactaaaaaa tctatctgtt tcttttcatt ctctgtattt tttatagttt1981ctgttgcatg ggcataaagt tgccttttta atcacaattc agaaaatatc ataatatctc2041atttcactaa ataatagtga acggcaggta tatgtgatgg gttaaaaagg atcgatcctc2101tagctagagt cgatcttcgc cagcagggcg aggatcgtgg catcaccgaa ccgcgccgtg2161cgcgggtcgt cggtgagcca gagtttcagc aggccgccca ggcggcccag gtcgccattg2221atgcgggcca gctcgcggac gtgctcatag tccacgacgc ccgtgatttt gtagccctgg2281ccgacggcca gcaggtaggc cgacaggctc atgccggccg ccgccgcctt ttcctcaatc2341gctcttcgtt cgtctggaag gcagtacacc ttgataggtg ggctgccctt cctggttggc2401ttggtttcat cagccatccg cttgccctca tctgttacgc cggcggtagc cggccagcct2461cgcagagcag gattcccgtt gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa2521cacccgctcg cgggtgggcc tacttcacct atcctgcccg gctgacgccg ttggatacac2581caaggaaagt ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa aaaggatgga2641tataccgaaa aaatcgctat aatgaccccg aagcagggtt atgcagcgga aaagcgctgc2701ttccctgctg ttttgtggaa tatctaccga ctggaaacag gcaaatgcag gaaattactg2761aactgagggg acaggcgaga gacgatgcca aagagctaca ccgacgagct ggccgagtgg2821gttgaatccc gcgcggccaa gaagcgccgg cgtgatgagg ctgcggttgc gttcctggcg2881gtgagggcgg atgtcgatat gcgtaaggag aaaataccgc atcaggcgca tatttgaatg2941tatttagaaa aataaacaaa aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg3001gccttctgct taatttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg3061ggccgttgct tcgcaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg3121ttcaccgaca aacaacagat aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt3181atttgatgcc tggcagttcc ctactctcgc atggggagac cccacactac catcggcgct3241acggcgtttc acttctgagt tcggcatggg gtcaggtggg accaccgcgc tactgccgcc3301aggcaaattc tgttttatca gaccgcttct gcgttctgat ttaatctgta tcaggctgaa3361aatcttctct catccgccaa aacagccaag ctcgccattc gccattcagg ctgcgcaact3421gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat3481gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa3541cgacggccag tgccaagctt gcatgcctgc aggtcgactc tagaggatcc ccgggtaccg3601agctcgaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca3661attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg3721agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg3781tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc3841tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta3901tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag3961aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg4021tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg4081tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg4141cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga4201agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc4261tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt4321aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact4381ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg4441cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt4501accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt4561ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct4621ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg4681gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt4741aaatcaatct aaagtatata tgagtaaact tggtctgaca atcgatgcaa gggttggttt4801gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt4861tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg4921caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca4981tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag5041gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct5101ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat5161ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc5221cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac5281gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt5341cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc5401tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc5461ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcggc gctctccctt5521atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc5581cgcaaggaat ggtgcatgta aggagatggc gcccaacagt cccccggcca cggggcctgc5641caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc5701atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc5761cacgatgcgt ccggcgtaga gaatccacag gacgggtgtg gtcgccatga tcgcgtagtc5821gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag5881tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc5941atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg6001cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc6061attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc6121gcattaaagc taatcggttg aatactcata ctcttccttt ttcaatatta ttgaagcatt6181tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa6241ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt6301atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtc//