FITC标记的Smad蛋白E3泛素连接酶2抗体
产品名称： FITC标记的Smad蛋白E3泛素连接酶2抗体
英文名称： Anti-SMURF2/FITC
产品编号： HZ-4056R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： Flow-Cyt=1:50-200 IF=1:50-200
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Rabbit Anti-SMURF2/FITC Conjugated antibody
FITC标记的Smad蛋白E3泛素连接酶2抗体
| 英文名称 | Anti-SMURF2/FITC |
| 中文名称 | FITC标记的Smad蛋白E3泛素连接酶2抗体 |
| 别 名 | hSMURF2; MGC138150; Smad specific E3 ubiquitin ligase 2; SMAD specific E3 ubiquitin protein ligase 2; Smad ubiquitination regulatory factor 2; Ubiquitin protein ligase SMURF2; DKFZp686F0270; MGC138150; E3 ubiquitin-protein ligase SMURF2; EC 6.3.2.; SMAD-specific E3 ubiquitin-protein ligase 2; SMUF2_HUMAN; Smurf2. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 肿瘤 细胞生物 免疫学 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Chicken, Cow, Horse, |
| 产品应用 | Flow-Cyt=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 82kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SMURF2 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: SMURF2, a 748-amino acid ubiquitin E3 ligase that is 83% identical to SMURF1, codes for a C2-WW-HECT domain ubiquitin ligase that associates constitutively with SMAD7. Binding to SMAD7 induces export of SMURF2 and recruitment to the activated transforming growth factor-beta receptor (TGFBR), where it causes receptor and SMAD7 degradation. A strong interaction of second and third SMURF2 WW domains has been identified with SMAD1, SMAD2, and SMAD3, but not SMAD4. Western blot analysis showed that SMURF2 selectively downregulates the transcription of SMAD2 and SMAD1, but not SMAD3. The nuclear SMURF2/phosphorylated SMAD2 interaction is requires TGFB1. Function: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level. Subunit: Interacts (via WW domains) with SMAD1. Interacts (via WW domains) with SMAD2 (via PY-motif). Interacts (via WW domains) with SMAD3 (via PY-motif). Interacts with SMAD6. Interacts with SMAD7 (via PY-motif) and TGFBR1; SMAD7 recruits SMURF2 to the TGF-beta receptor and regulates its degradation. Does not interact with SMAD4; SMAD4 lacks a PY-motif. Interacts with AIMP1. Interacts with STAMBP and RNF11. Interacts with NDFIP1 and NDFIP2 (Probable); this interaction activates the E3 ubiquitin-protein ligase. Subcellular Location: Nucleus. Cytoplasm. Cell membrane. Membrane raft. Cytoplasmic in the presence of SMAD7. Co-localizes with CAV1, SMAD7 and TGF-beta receptor in membrane rafts. Tissue Specificity: Widely expressed. Post-translational modifications: Auto-ubiquitinated and ubiquitinated in the presence of RNF11 and UBE2D1. Ubiquitinated by the SCF(FBXL15) complex, leading to its degradation by the proteasome. Similarity: Contains 1 C2 domain. Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain. Contains 3 WW domains. Database links: Entrez Gene: 64750 Human Entrez Gene: 66313 Mouse Entrez Gene: 303614 Rat Omim: 605532 Human SwissProt: Q52LL1 Human SwissProt: Q9HAU4 Human SwissProt: A2A5Z6 Mouse SwissProt: Q3TT87 Mouse SwissProt: Q5IRE6 Mouse Unigene: 340955 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |
SMRF2是748个氨基酸泛素E3连接酶，与SMRUF1的83%相同，编码C2-WW-HECT结构域泛素连接酶，其与构成SMAD7结合。结合SMAD7诱导SMRF2的出口和招聘到激活的转化生长因子-β受体（TGFBR），在那里它会导致受体和SMAD7降解。Smad1、Smad2和Smad3与第二和第三SmadR2 WW结构域的强相互作用已被鉴定，但不是Smad4。Western blot分析表明，SMRU2选择性下调SMAD2和SMAD1的转录，而不下调SMAD3的转录。核SMRF2/磷酸化SMAD2相互作用需要TGFB1。