分子量:17.4 kDa

纯度:>95% as determined by reduced SDS-PAGE. 

内毒素:Less than 1.0 EU per μg of the Biotinylated Human TNF-alpha, Superior Sensitivity by the LAL method.

Buffer:PBS, pH7.4

生物活性:Measured by its binding ability in a functional ELISA. 
Immobilized Humira at 5 g/mL (100 l/well) can bind Biotinylated Human TNF-alpha, Superior Sensitivity (Catalog # TNA-H821R) with a linear range of 0.4-3 ng/mL.

产品特性:Biotinylated Human TNF-alpha Protein, Tag Free, Superior Sensitivity  has a calculated MW of 17.4 kDa. The predicted N-terminus is Val 77. The reducing (R) protein migrates as 18 kDa in SDS-PAGE.

产品背景:Tumor necrosis factor alpha (TNFα)  is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimer's disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin. TNFα can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.