KAMIYA BIOMEDICAL COMPANY
Rev. 102902
PRODUCT DATA SHEET
KAMIYA BIOMEDICAL COMPANY, Seattle, WA USA Tel: (206) 575-8068 • Fax: (206) 575-8094
www.kamiyabiomedical.com techsupport@kamiyabiomedical.com
Product: Anti-DFF40 / CAD (I18), Polyclonal
Cat. No: PC-150 (100 μg)
Description:
Deregulation of apoptosis is related to a variety
of diseases. Apoptosis can be induced by a
family of cell death receptors and their ligands.
Death signals are transduced by death domain
containing adapter molecules and members of
the caspase family of proteases. These death
signals finally cause the degradation of
chromosomal DNA by activated Dnase. A
mouse Dnase that causes DNA fragmentation
was identified recently and designated CAD (for
caspase activated deoxyribonuclease). The
human homolog of mouse CAD was termed
CPAN, DFF40, and human CAD, respectively.
DFF45(ICAD) is the inhibitory protein of
DFF40(CAD). Upon cleavage of DFF45(ICAD)
by activated caspase, DFF40(CAD) is released
from the DFF45(ICAD) complex and activated
and eventually causes the degradation of DNA
in the nuclei. Activation of DFF40(CAD) causes
DNA degradation, which is the mark of apoptotic
cell death.
Specificity:
Recognizes human, mouse, and rat DFF40 /
CAD. Detects a band of ~40 kDa by Western
blot.
Species Reactivity:
Human, mouse, and rat, other species not
tested.
Ig Isotype:
Rabbit IgG
Immunogen:
Synthetic peptide corresponding to aa 147-164
(E147GLESRFRNKSGYLRYS164) of mouse
caspase activated deoxyribonuclease (CAD).
The sequence differs from human DFF40 by two
amino acids.
Format:
100 μg of purified rabbit IgG in 200 μl PBS
containing 0.02% sodium azide.
Storage and Stability:
Store at +4°C.
Applications and Suggested Dilutions:

Western blot: 1:500 – 1:1,000
The optimal dilution for a specific application
should be determined by the researcher.
Figure: Western blot analysis of DFF40/CAD in
HeLa (H), K562 (K), Jurkat (J), and Raji (R)
whole cell lysate with anti-DFF40/CAD (I18) at
1:500 dilution.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.