分子量:20 kDa

纯度:>95% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human CD40, His Tag by the LAL method.

Buffer:PBS, pH7.4

生物活性:Measured by its binding ability in a functional ELISA. Immobilized Human CD40, His Tag at 1 g/mL (100 L/well) can bind Human CD40 Ligand /TNFSF5 with a linear range of 0.01-0.12 ng/mL.

产品特性:Human CD40, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 20 kDa. The predicted N-terminus is Glu 21. The reducing (R) protein migrates as 32 kDa in SDS-PAGE  due to glycosylation.

产品背景:Tumor necrosis factor receptor superfamily member 5 (TNFRSF5) is also known as CD40, is a member of the TNF receptor superfamily. The expression of CD40 is diverse. TNFRSF5 has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 is the receptor for TNFSF5/CD40LG. Defects in CD40 are the cause of immunodeficiency with hyper-IgM type 3 (HIGM3).