ASPP2,P53凋亡刺激蛋白2抗体

产品编号HZ-1283R
英文名称ASPP2
中文名称P53凋亡刺激蛋白2抗体
别 名Apoptosis-stimulating of p53 protein 2; Bbp; Bcl2-binding protein; NY-REN-51 antigen; p53-binding protein 2; p53BP2; PPP1R13A; TP53BP2; Tumor suppressor p53-binding protein 2; apoptosis stimulating protein of P53 2; ASPP2_HUMAN.
规格价格0.1ml 0.2ml
说 明 书0.1ml 0.2ml
研究领域肿瘤 细胞生物 免疫学 细胞凋亡 转录调节因子
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Chicken,
ASPP2,P53凋亡刺激蛋白2抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量124kDa
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human ASPP2
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
ASPP2,P53凋亡刺激蛋白2抗体PubMedPubMed
产品介绍background:
This gene encodes a member of the ASPP (apoptosis-stimulating protein of p53) family of p53 interacting proteins. The protein contains four ankyrin repeats and an SH3 domain involved in protein-protein interactions. It is localized to the perinuclear region of the cytoplasm, and regulates apoptosis and cell growth through interactions with other regulatory molecules including members of the p53 family. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Function:
Regulator that plays a central role in regulation of apoptosis and cell growth via its interactions. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo. Inhibits the ability of APPBP1 to conjugate NEDD8 to CUL1, and thereby decreases APPBP1 ability to induce apoptosis. Impedes cell cycle progression at G2/M. Its apoptosis-stimulating activity is inhibited by its interaction with DDX42.

Subunit:
Binds to the central domain of TP53 as well as to BCL2. Interacts with protein phosphatase 1. Interacts with RELA NF-kappa-B subunit. This interaction probably prevents the activation of apoptosis, possibly by preventing its interaction with TP53. Interacts with APC2 and APPBP1. Interacts with DDX42 (via the C-terminus); the interaction is not inhibited by TP53BP2 ubiquitination and is independent of p53/TP53.

Subcellular Location:
Cytoplasm, perinuclear region. Nucleus. Note=Predominantly found in the perinuclear region. Some small fraction is nuclear. Sequester in the cytoplasm on overexpression of DDX42.

Tissue Specificity:
Widely expressed. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocyte. Reduced expression in breast carcinomas expressing a wild-type TP53 protein. Overexpressed in lung cancer cell lines.

ASPP2,P53凋亡刺激蛋白2抗体Similarity:
Belongs to the ASPP family.
Contains 2 ANK repeats.
Contains 1 SH3 domain.

Gene ID:
7159

Database links:
Entrez Gene: 7159 Human
Entrez Gene: 209456 Mouse
Omim: 602143 Human
SwissProt: Q13625 Human
SwissProt: Q8CG79 Mouse
Unigene: 523968 Human
Unigene: 287450 Mouse

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

p53凋亡刺激蛋白(apoptosis stimulating protein of p53，ASPP)家族是近年来发现的一个新蛋白家族，它包括3个成员：ASPP1、ASPP2和iASPP。其中ASPP1和ASPP2能够与p53蛋白结合增强该蛋白的促进细胞凋亡的功能，发挥抑制肿瘤的作用；而iASPP与ASPP1和ASPP2的功能截然相反，它能够和ASPP1或ASPP2竞争性地与p53结合，抑制ASPP1或ASPP2的促进凋亡作用，具有癌基因的功能。三者的结构相似，但功能却完全不同，通过研究三者的关系、促进肿瘤细胞内ASPP1或ASPP2的高表达以及抑制iASPP的表达有可能促进肿瘤细胞的凋亡，成为肿瘤治疗的新靶点.