Product: Anti-BAFF, FITC labeled, clone Buffy-2
Cat. No.: MC-109 (100 test)
Synonyms:
BLyS, TALL-1, THANK, zTNF4, TNFSF 13B/20,
CD257.
Specificity:
Recognizes membrane-bound and soluble
human and mouse (weak) BAFF.
Species Reactivity:
Human and mouse (weak). Others species not
tested.
Ig Isotype:
Rat IgM
Immunogen:
Recombinant human soluble BAFF (aa 83-285).
Schematic structure of rhsBAFF (aa. 83-285). Antibody to
BAFF (MC-109) recognizes the TNF-homology domain.
Format:
100 μg of antibody at 1 mg/mL in PBS with
0.02% sodium azide. >95% purity as determined
by SDS-PAGE.
Storage:
Store at 4°C.
Applications:

Flow cytometry
The optimal dilution for a specific application
should be determined by the researcher.
Figure: FITC labeled monoclonal antibody to BAFF (MC-
109) detects membrane-bound human BAFF by FACS.
Method: HEK 293T cells (5 x 105) were mock transfected
(thin line) or transfected with an expression plasmid
enabling surface expression of full length hBAFF (thick
line). Cells were incubated on ice for 30 min in 50μL FACS
buffer (PBS, 5% fetal calf serum, 0.02% azide) containing
1 μg/mL of FITC labeled antibody to BAFF (MC-109). After
washing, cells were analyzed by flow cytometry.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product