水通道蛋白-3抗体，AQP3抗体

产品编号HZ-1253R
英文名称AQP3
中文名称水通道蛋白-3抗体
别 名AQP 3; AQP3; Aquaporin 3 (GIL blood group); Aquaporin 3 (Gill blood group); Aquaporin3; Aquaporin-3; GIL; Gill blood group; AQP3_MOUSE.
说 明 书0.1ml 0.2ml
研究领域肿瘤 信号转导 通道蛋白
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat,
产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量32kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from mouse AQP3 C-terminus
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
水通道蛋白-3抗体，AQP3抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
This gene encodes the water channel protein aquaporin 3. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein, also known as aquaporin 0. Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transport of nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms. [provided by RefSeq, Aug 2011]

Function:
Water channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism.

Subcellular Location:
Membrane; Multi-pass membrane protein.

Tissue Specificity:
Renal medulla and colon. Predominantly in the inner medulla. Expressed in basal layer of epidermal keratinocytes.

Similarity:
Belongs to the MIP/aquaporin (TC 1.A.8) family.

Gene ID:
11828

Database links:
Entrez Gene: 360 Human
Entrez Gene: 11828 Mouse
Entrez Gene: 65133 Rat
Omim: 600170 Human
SwissProt: Q92482 Human
SwissProt: Q8R2N1 Mouse
SwissProt: P47862 Rat
Unigene: 234642 Human
Unigene: 34043 Mouse
Unigene: 11109 Rat
Important Note:
水通道蛋白-3抗体，AQP3抗体This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

通道蛋白（Channel Protein）
AQP 3主要分布于肾脏集合管上皮细胞基侧质膜,除了对水有通透性外、还可转运尿素和甘油等小分子物质,而尿素在尿液浓缩机制中有着重要的作用.
有学者认为：AQP-3的阳性率与某些肿瘤分化程度有关（胃癌、结肠癌、乳腺癌、前列腺癌、子宫癌、卵巢癌、甲状腺癌等）
产品图片
水通道蛋白-3抗体，AQP3抗体Tissue/cell: rat taste buds; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-AQP-3 Polyclonal Antibody, Unconjugated(bs-1253R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining