ASBT,顶膜钠依赖性胆盐转运体蛋白抗体

产品编号HZ-4189R
英文名称ASBT
中文名称顶膜钠依赖性胆盐转运体蛋白抗体
别 名SLC10A2; Apical sodium dependent bile acid transporter; Apical sodium- dependent bile acid transporter; IBAT; ileal apical sodium-dependent bile acid transporter; Ileal sodium dependent bile acid transporter; Ileal sodium-dependent bile acid transporter; Ileal sodium/bile acid cotransporter; ISBT; Na+ bile acid cotransporter; Na+ dependent ileal bile acid transporter; NTCP2; Sodium/taurocholate cotransporting polypeptide, ileal; solute carrier family 10 (sodium/bile acid cotransporter family); Solute carrier family 10 member 2.
规格价格0.1ml 0.2ml 
说 明 书0.1ml 0.2ml
研究领域肿瘤 免疫学 信号转导 通道蛋白
抗体来源Rabbit
ASBT,顶膜钠依赖性胆盐转运体蛋白抗体克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Dog, Horse,
产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量38kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human ASBT
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
ASBT,顶膜钠依赖性胆盐转运体蛋白抗体PubMedPubMed
产品介绍background:
SLC10A2 plays a critical role in reabsorption of bile acids from the the small intestine lumen. Passive flow of sodium ions down their concentration gradient is coupled to bile acid movement, resulting in an increase in the concentration of bile acids in the interior of the cell. This action conserves the body's pool of re-circulating bile acid. SLC10A2 also plays a key role in cholesterol metabolism as cholesterol is the precursor molecule in bile acid synthesis mediated by CYP7A and FXR.

Function:
Plays a critical role in the sodium-dependent reabsorption of bile acids from the lumen of the small intestine. Plays a key role in cholesterol metabolism.

Subunit:
Monomer and homodimer.

Subcellular Location:
Membrane; Multi-pass membrane protein.

DISEASE:
Primary bile acid malabsorption (PBAM) [MIM:613291]: An intestinal disorder associated with chronic watery diarrhea, excess fecal bile acids, steatorrhea and interruption of the enterohepatic circulation of bile acids. {ECO:0000269|PubMed:9109432}. Note=The disease is caused by mutations affecting the gene represented in this entry.

Similarity:
Belongs to the bile acid:sodium symporter (BASS) (TC 2.A.28) family.

Gene ID:
6555

ASBT,顶膜钠依赖性胆盐转运体蛋白抗体Database links:
Entrez Gene: 6555 Human
Entrez Gene: 20494 Mouse
Entrez Gene: 29500 Rat
Omim: 601295 Human
SwissProt: Q12908 Human
SwissProt: P70172 Mouse
SwissProt: Q62633 Rat
Unigene: 194783 Human
Unigene: 3500 Mouse
Unigene: 85891 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品介绍

background: SLC10A2 plays a critical role in reabsorption of bile acids from the the small intestine lumen. Passive flow of sodium ions down their concentration gradient is coupled to bile acid movement, resulting in an increase in the concentration of bile acids in the interior of the cell. This action conserves the body's pool of re-circulating bile acid. SLC10A2 also plays a key role in cholesterol metabolism as cholesterol is the precursor molecule in bile acid synthesis mediated by CYP7A and FXR. Function: Plays a critical role in the sodium-dependent reabsorption of bile acids from the lumen of the small intestine. Plays a key role in cholesterol metabolism. Subunit: Monomer and homodimer. Subcellular Location: Membrane; Multi-pass membrane protein. DISEASE: Primary bile acid malabsorption (PBAM) [MIM:613291]: An intestinal disorder associated with chronic watery diarrhea, excess fecal bile acids, steatorrhea and interruption of the enterohepatic circulation of bile acids. {ECO:0000269|PubMed:9109432}. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the bile acid:sodium symporter (BASS) (TC 2.A.28) family.  Gene ID: 6555 Database links:   Entrez Gene: 6555 Human Entrez Gene: 20494 Mouse Entrez Gene: 29500 Rat Omim: 601295 Human SwissProt: Q12908 Human SwissProt: P70172 Mouse SwissProt: Q62633 Rat Unigene: 194783 Human Unigene: 3500 Mouse Unigene: 85891 Rat     Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片

Tissue/cell: mouse intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-ASBT Polyclonal Antibody, Unconjugated(bs-4189R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ASBT) Polyclonal Antibody, Unconjugated (bs-4189R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ASBT) Polyclonal Antibody, Unconjugated (bs-4189R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ASBT) Polyclonal Antibody, Unconjugated (bs-4189R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.