分子量:54.7 kDa

纯度:>95% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human CNDP1, His Tag by the LAL method.

Buffer:50 mM Tris, 150 mM NaCl, pH 7.4

生物活性:Measured by its ability to cleave carnosine ( beta -Ala-L-His) in a two-step assay. The specific activity is >250 pmol/min/g.

产品特性:Human CNDP1, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 54.7 kDa. The predicted N-terminus is Ser 28. The reducing (R) protein migrates as 60-66 kDa in SDS-PAGE  due to glycosylation.

产品背景:Beta-Ala-His dipeptidase is also known as Carnosine dipeptidase 1 (CNDP1), CNDP dipeptidase 1, Glutamate carboxypeptidase-like protein 2, Serum carnosinase, CPGL2, which belongs to the peptidase M20A family. CNDP1 is found in serum and adult nervous central system. CNDP1 preferential hydrolysis of the beta-Ala-|-His dipeptide (carnosine), and also anserine, Xaa-|-His dipeptides and other dipeptides including homocarnosine. The catalytic activity of CNDP1 is inhibited by the metal chelator 1,10-o-phenantrolin and the inhibitory concentration 50% (IC50) is 5 M.