Endomucin,内皮粘蛋白EMCN抗体

产品编号HZ-4808R
英文名称Endomucin
中文名称内皮粘蛋白EMCN抗体
别 名EMCN; EMCN2; Endomucin 2; Endomucin; Endomucin-2; Endothelial sialomucin; Gastric cancer antigen Ga34; MUC-14; MUCEN_HUMAN; Mucin 14; Mucin-14.
说 明 书0.1ml 0.2ml
研究领域肿瘤 免疫学 信号转导 糖蛋白 细胞骨架 细胞外基质
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human,
Endomucin,内皮粘蛋白EMCN抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量25kDa
细胞定位细胞膜 分泌型蛋白
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from hu bs-4808R
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Endomucin,内皮粘蛋白EMCN抗体PubMedPubMed
产品介绍background:
Endothelial sialomucin, also called endomucin or mucin-like sialoglycoprotein, which interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix.

Function:
Endothelial sialomucin, also called endomucin or mucin-like sialoglycoprotein, which interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix.

Subcellular Location:
Isoform 1: Cell membrane; Single-pass type I membrane protein. Membrane; Single-pass type I membrane protein. Note=Consistently localized to the plasma membrane and less abundantly to Golgi-like perinuclear stacks.
Isoform 2: Secreted (Potential).

Tissue Specificity:
Expressed in heart, kidney and lung.

Post-translational modifications:
Highly O-glycosylated. Sialic acid-rich glycoprotein.

Database links:
Entrez Gene: 51705 Human
Omim: 608350 Human
SwissProt: Q9ULC0 Human
Unigene: 152913 Human

Endomucin,内皮粘蛋白EMCN抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-Endomucin Polyclonal Antibody, Unconjugated(HZ-4808) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: mouse tumor tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-Endomucin Polyclonal Antibody, Unconjugated(HZ-4808) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining