phospho-BNIP3 (Ser95),磷酸化促凋亡调节蛋白BNIP3抗体

产品编号HZ-12875R
英文名称phospho-BNIP3 (Ser95)
中文名称磷酸化促凋亡调节蛋白BNIP3抗体
别 名BNIP3 (phospho S95); p-BNIP3 (phospho S95); BCL2 Adenovirus E1B 19kDa Interacting Protein 3; BCL2/adenovirus E1B 19 kDa protein interacting protein 3; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3; BNIP 3; BNIP3; BNIP3_HUMAN; NIP 3; NIP3.
说 明 书0.1ml
产品类型磷酸化抗体
研究领域肿瘤 细胞生物 细胞凋亡 新陈代谢
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Dog, Cow, Horse, Sheep,
phospho-BNIP3 (Ser95),磷酸化促凋亡调节蛋白BNIP3抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量22kDa
细胞定位细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthesised phosphopeptide derived from human BNIP3 around the phosphorylation site of Ser95
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
phospho-BNIP3 (Ser95),磷酸化促凋亡调节蛋白BNIP3抗体PubMedPubMed
产品介绍background:
The adenovirus E1B protein is a viral homolog of the Bcl-2 family of proteins that are involved in regulating cell death. A family of interacting proteins, which are designated Nip or Bnip and include BNIP-1, BNIP-2, BNIP-3 and Nix, associate with both the E1B protein and Bcl-2 proteins to mediate apoptotic signaling. BNIP-1 contains a hydrophobic transmembrane domain, which enables its localization to the nuclear envelope, endoplasmic recticulum and mitochondria. BNIP-2, (previously designated Nip2 and Nip21 in human and mouse respectively), shares homology with the non-catalytic domain of Cdc42 GTPase-activating protein (Cdc42GAP). Through binding to Cdc42GAP, BNIP-2 enhances the GTPase activity of Cdc42GAP, facilitating the hydrolysis of GTP bound to Cdc42 and thereby, mediating the signaling pathways involving receptor kinases, small GTPases and apoptotic proteins. Nix, which is also designated Nip3L or Bnip3L, is highly related to BNIP-3, and both proteins localize to the mitochondria where they associate with Bcl-2 proteins. BNIP-3 preferentially binds to Bcl-xL and induces apoptosis by suppressing the anti-apoptosis activity of Bcl-xL.

Function:
Apoptosis-inducing protein that, which can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2.

Subunit:
Homodimer. Binds to BCL2. Interacts with BNIP3L and ACAA2. Also can interact with adenovirus E1B 19 kDa protein or Epstein-Barr virus BHRF1. Interacts (via BH3 domain) with SPATA18 (via coiled-coil domains).

phospho-BNIP3 (Ser95),磷酸化促凋亡调节蛋白BNIP3抗体Subcellular Location:
Mitochondrion. Mitochondrion membrane. Coexpression with the EIB 19-kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope. Colocalizes with ACAA2 in the mitochondria.

Post-translational modifications:

Similarity:
Belongs to the NIP3 family.

Database links:
UniProtKB/Swiss-Prot: Q12983.2

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: human liver carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-phospho-BNIP3(Ser95)Polyclonal Antibody, Unconjugated(HZ-12875R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining