Oligophrenin 1,精神发育迟滞相关蛋白Oligophrenin-1抗体

产品编号HZ-11469R
英文名称Oligophrenin 1
中文名称精神发育迟滞相关蛋白Oligophrenin-1抗体
别 名Oligophrenin1; Oligophrenin-1; OPHN1_HUMAN.
说 明 书0.1ml 0.2ml
研究领域细胞生物 神经生物学 信号转导 G蛋白信号
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Cow, Horse, Sheep,
Oligophrenin 1,精神发育迟滞相关蛋白Oligophrenin-1抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量92kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from huamn Oligophrenin 1 (418-465aa)
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Oligophrenin 1,精神发育迟滞相关蛋白Oligophrenin-1抗体PubMedPubMed
产品介绍background:
Ras p21 can exist in either a physiologically quiescent GDP-binding state or a GTP-binding signal-emitting state (1,2). Interaction of Ras p21 with GTPase activating protein (GAP) can increase the rate of hydrolysis of Ras p21-bound GTP by as much as 1000-fold (3). In mitogenically activated and tyrosine kinase-transformed cells, Ras GAP forms a complex with a protein designated p190 (4). At its amino terminus, p190 contains sequence motifs characteristic of all known GTPases, whereas the carboxy terminus contains sequences similar to those found in the Bcr gene product, n-chimerin and Rho GAP, all of which exhibit intrinsic GAP activity (4,5). Oligophrenein-1 is an additional protein with GTPase activating activity. Oligophrenein-1 is a RhoGAP protein that stimulates GTP hydrolysis of Rho subfamily members and is involved in cell migration, morphogenesis and axon outgrowth (6).

Function:
Stimulates GTP hydrolysis of members of the Rho family. Could activates GTPase targets that are known to affect cell migration and outgrowth of axons and dendrites.

Subunit:
Interacts with HOMER1. Interacts with AMPA receptor complexes. Interacts with SH3GL2 (endophilin-A1)

Subcellular Location:
Cell junction, synapse. Cell projection, axon. Cell projection, dendritic spine. Note=Present in both presynaptic and postsynaptic sites

Tissue Specificity:
Expressed in brain.

Oligophrenin 1,精神发育迟滞相关蛋白Oligophrenin-1抗体DISEASE:
Defects in OPHN1 are the cause of mental retardation X-linked OPHN1-related (MRXSO) [MIM:300486]; formerly designated MRX60. MRXSO is a syndromic mental retardation. Patients present mental retardation associated with cerebellar hypoplasia and distinctive facial dysmorphism.

Similarity:
Contains 1 PH domain.
Contains 1 Rho-GAP domain.

Gene ID:
4983

Database links:
Entrez Gene: 4983 Human
Omim: 300127 Human
SwissProt: O60890 Human

Oligophrenin 1,精神发育迟滞相关蛋白Oligophrenin-1抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-Oligophrenin 1 Polyclonal Antibody, Unconjugated(HZ-11469R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining