UBE2O,泛素结合酶E2 230K抗体

产品编号HZ-8380R
英文名称UBE2O
中文名称泛素结合酶E2 230K抗体
别 名E2 230K; ubiquitin-conjugating enzyme E2O; EC=6.3.2.19; FLJ12878; KIAA1734; Likely Ortholog of Mouse Ubiquitin Conjugating Enzyme; RP23 193A16.5; UBE2O; UBE2O_HUMAN; Ubiquitin carrier protein O; Ubiquitin conjugating enzyme E2 O; ubiquitin conjugating enzyme E2O.
说 明 书0.1ml 0.2ml
研究领域细胞生物 免疫学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Mouse, Rat, Chicken, Pig, Horse,
UBE2O,泛素结合酶E2 230K抗体产品应用ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量141kDa
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human UBE2O/E2 230K
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
UBE2O,泛素结合酶E2 230K抗体PubMedPubMed
产品介绍background:
UBE20, also known as E2-230K, is a 1,292 amino acid member of the uniquitin-conjugating enzyme family that is involved in protein modification. Expressed predominately in heart and skeletal muscle, UBE2O functions to catalyze the ATP-dependent covalent attachment of ubiquitin to select proteins, thereby targeting the ubiquitinated proteins for proteasomal degradation. The gene encoding UBE2O maps to human chromosome 17, which comprises over 2.5% of the human genome and encodes over 1,200 genes. Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1. Tumor suppressor p53 is necessary for maintenance of cellular genetic integrity by moderating cell fate through DNA repair versus cell death. Malfunction or loss of p53 expression is associated with malignant cell growth and Li-Fraumeni syndrome. Like p53, BRCA1 is directly involved in DNA repair, though specifically it is recognized as a genetic determinant of early onset breast cancer and predisposition to cancers of the ovary, colon, prostate gland and fallopian tubes.

Function:
Catalyzes the covalent attachment of ubiquitin to other proteins (By similarity).

Tissue Specificity:
Predominantly expressed in skeletal muscle and heart.

Similarity:
Belongs to the ubiquitin-conjugating enzyme family.

Gene ID:
63893

Database links:
Entrez Gene: 63893 Human
Entrez Gene: 217342 Mouse
SwissProt: Q9C0C9 Human
SwissProt: Q6ZPJ3 Mouse
Unigene: 16130 Human
Unigene: 243950 Mouse

UBE2O,泛素结合酶E2 230K抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片	Sample:A549 Cell (Human) Lysate at 40 ug Primary: Anti-UBE2O (HZ-8380R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 141kD Observed band size: 48kD