• 靶标种属Mouse
• 特异性By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
• 经测试应用ICC, IHC-P, ELISA, WBmore details
• 偶联物HRP

性能

• 形式Liquid
• 存放说明Shipped at 4°C. Store at +4°C.
• 存储溶液Preservative: 0.1% Proclin
  Constituents: 0.2% BSA, PBS
• 浓度1 mg 浓度为 1 mg/ml 
  
• 纯度Immunogen affinity purified
• 纯化说明This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
• 共轭说明Molar enzyme/ antibody protein ratio is 4:1
• 克隆多克隆
• 同种型IgG
• 常规说明Part of the AbExcel range.
• 研究领域
  • Immunology
   
  • Immunoglobulins
   
  • Heavy Chain
   
  • IgG

  • Secondary antibodies
   
  • anti-Mouse
   
  • IgG
   
  • Enzyme
   
  • HRP

应用

Rabbit Anti-Mouse IgG H&L (HRP) 图像

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Mouse IgG H&L (HRP) (ab97046)

  IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8224, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (ab97046, 1/500 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

  The inset negative control image is taken from an identical assay without primary antibody.

  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• 

  Western blot - Rabbit anti-Mouse IgG H&L (HRP) secondary antibody (ab97046)

  All lanes : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1 µg/ml Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane. Secondary Lanes 1 - 2 : Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab97046) at 1/2000 dilution Lanes 3 - 4 : Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab97046) at 1/10000 dilution Lanes 5 - 6 : Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab97046) at 1/20000 dilution Developed using the

• 

  Western blot - Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab97046)
  
  developed using the ECL technique
  
  Performed under non-reducing conditions.
  
  Exposure time : 2 seconds

  Sample: Human Cell lysate - whole cell (human glioma cells), Loading amount: 20 µg, Specification: human glioma cells

  Blocking step: Milk as blocking agent for 1 hour, Concentration: 5 %, Temperature: 25°C

  Other product details: 1st Ab Dilution 1/5000 anti-actin Ab, Incubation time: 16 hours, Temperature: 4°C

  Secondary antibody: ab97046 used at 1:20,000

  Bands: Specific: 45 kDa

  Notes: primary Ab (anti-actin) was used as a loading control in 6 human glioma cell lines

  This image is courtesy of an anonymous Abreview