Trimethyl-Histone H3 polyclonal antibody
产品名称： Trimethyl-Histone H3 polyclonal antibody
英文名称： Trimethyl-Histone H3 polyclonal antibody
产品编号： PAB12741
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
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- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of Trimethyl-Histone H3.
- Immunogen:
- A synthetic peptide corresponding to trimethyl lysine 4 of human Histone H3.
- Host:
- Rabbit
- Theoretical MW (kDa):
- 17
- Reactivity:
- Bovine, Chicken, Human, Mouse, Rat
- Specificity:
- This antibody only recognizes ~ 17 KDa of Trimethylated Histone H3 at K4. It does not cross react to non-methylated H3 or mono-, di-methylated Histone H3.
- Form:
- Liquid
- Storage Buffer:
- In PBS, pH 7.2 (10% Proclin300)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (1:100)
The optimal working dilution should be determined by the end user.
- Publication Reference
- 1.
- Demethylation of trimethylated histone H3 Lys4 in vivo by JARID1 JmjC proteins.
Seward DJ, Cubberley G, Kim S, Schonewald M, Zhang L, Tripet B, Bentley DL.Nat Struct Mol Biol. 2007 Mar;14(3):240-2. Epub 2007 Feb 18.
- 2.
- Histone H3 lysine 9 methylation and HP1gamma are associated with transcription elongation through mammalian chromatin.
Vakoc CR, Mandat SA, Olenchock BA, Blobel GA.Mol Cell. 2005 Aug 5;19(3):381-91.
- Applications
- Immunofluorescence
- Fluorescent Immunohistochemistry : NIH/3T3 cells were cultured in chamber, and fixed onto slide followed by incubation with Trimethyl-Histone H3 polyclonal antibody (Cat # PAB12741) at 1 : 100 for 10 min at RT. FITC labeled Gt anti Rb IgG was used for visualization under fluorescent microscope.
- Immunoprecipitation
- The nuclear extract derived from NIH/3T3 were immunoprecipitated by 4 ug of Trimethyl-Histone H3 polyclonal antibody (Cat # PAB12741) (Lane 2) and a rabbit IgG as a control (Lane 1), then probed with Trimethyl-Histone H3 polyclonal antibody (Cat # PAB12741) at 1 : 1000.
An immunoreactive band at ~17 KDa is observed.
Note: ~50 KDa and ~22 KDa are the rabbit immunoglobulin heavy and light chain.