DNA Polymerase delta, catalytic subunitDNA聚合酶δ催化亚单位/DNA pol δ cat抗体

产品编号HZ-13015R
英文名称DNA Polymerase delta, catalytic subunit
中文名称DNA聚合酶δ催化亚单位/DNA pol δ cat抗体
别 名CDC2; CDC2 homolog; DNA directed DNA polymerase delta 1; DNA directed polymerase delta 1; DNA pol delta 1; DNA polymerase delta catalytic subunit; DNA polymerase subunit delta p125; DPOD1_HUMAN; POLD; POLD 1; POLD1; Polymerase (DNA directed) delta 1 catalytic subunit 125kDa; Polymerase DNA directed delta 1 catalytic subunit 125kD.
说 明 书0.1ml 0.2ml
研究领域细胞生物 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Dog, Pig,
DNA Polymerase delta, catalytic subunitDNA聚合酶δ催化亚单位/DNA pol δ cat抗体产品应用IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量124kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human DNA Polymerase delta, catalytic subunit
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
DNA Polymerase delta, catalytic subunitDNA聚合酶δ催化亚单位/DNA pol δ cat抗体PubMedPubMed
产品介绍background:
DNA replication, recombination and repair, all of which are necessary for genome stability, require the presence of exonucleases (1). In DNA replication, these enzymes are involved in the processing of Okazaki fragments, whereas in DNA repair, they function to excise damaged DNA fragments and correct recombinational mismatches (2). Exonucleases involved in these processes include DNA polymerases, including DNA pol ∂ and é. DNA pol ∂ consists of two subunits, p125 which interacts directly with the sliding DNA clamp protein PCNA, and p50 (3,4). DNA pol ∂ can be regulated by cell cycle proteins (5). DNA pol é is a multiple subunit enzyme, the catalytic subunit of which is encoded by the POL2 gene (6,7). The exact reactions catalyzed by DNA pol ∂ and é on leading and lagging strands have not yet been elucidated.

Function:
Possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Required with its accessory proteins (proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) or activator 1) for leading strand synthesis. Also involved in completing Okazaki fragments initiated by the DNA polymerase alpha/primase complex.

Subunit:
Heterotetramer composed of subunits of 125 kDa, 50 kDa, 66 kDa and 12 kDa. The 125 kDa subunit contains the polymerase active site and most likely the active site for the 3'-5' exonuclease activity. Interacts with WRNIP1. Interacts with POLD4 and PCNA.

DNA Polymerase delta, catalytic subunitDNA聚合酶δ催化亚单位/DNA pol δ cat抗体Subcellular Location:
Nucleus.

Similarity:
Belongs to the DNA polymerase type-B family.
Contains 1 CysA-type zinc finger.

Gene ID:
5424

Database links:
Entrez Gene: 5424 Human
Entrez Gene: 18971 Mouse
Omim: 174761 Human
SwissProt: P28340 Human
SwissProt: P52431 Mouse
Unigene: 279413 Human
Unigene: 16549 Mouse

DNA Polymerase delta, catalytic subunitDNA聚合酶δ催化亚单位/DNA pol δ cat抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.